The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/1. Ready-to-use for 30 min at RT. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution (PMID 18183291).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPart of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Tissue specificityKeratinocytes of epidermis and other stratified squamous epithelia.
Sequence similaritiesBelongs to the involucrin family.
Post-translational modificationsSubstrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
Cellular localizationCytoplasm. Constituent of the scaffolding of the cornified envelope.