Anti-Involucrin antibody [SY5] (Biotin) (ab79031)

Overview

  • Product nameAnti-Involucrin antibody [SY5] (Biotin)See all Involucrin primary antibodies ...
  • Description
    Mouse monoclonal [SY5] to Involucrin (Biotin)
  • ConjugationBiotin
  • Tested applicationsWB, IP, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human, Pig, Monkey, Gorilla
  • Immunogen

    Human keratinocyte Involucrin

  • EpitopeCodon 421-568.
  • Positive control
    • LS174T cell lysate; Skin tissue.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab79031 is purified from ascites fluid by Protein G chromatography.
  • Clonality Monoclonal
  • Clone numberSY5
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab79031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ELISA
IHC-P
ICC/IF
  • Application notesELISA: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 1 - 2 µg/ml for 30 min at RT. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 10 min at 37°C or Protease XXV at 1mg/ml PBS for 5 minutes at 37°C.
    IP: Use at 2µg/mg of lysate (denatured).
    WB: Use at a concentration of 1 - 2 µg/ml for 2hrs at RT. Predicted molecular weight: 68 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Involucrin antibody [SY5] (Biotin) images

    • ab79031 at 2µg/ml staining Involucrin in human skin by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining in the epidermal region. The purified form of this antibody was used for staining.
    • anti-Involucrin at 2 µg/ml + LS174T cell lysate

      Predicted band size : 68 kDa

    References for Anti-Involucrin antibody [SY5] (Biotin) (ab79031)

    ab79031 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"