Overview

  • Product nameAnti-Involucrin antibody [SY8]
    See all Involucrin primary antibodies
  • Description
    Mouse monoclonal [SY8] to Involucrin
  • SpecificityKeratinocytes of epidermis and other stratified squamous epithelia
  • Tested applicationsSuitable for: IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Pig, Gorilla
  • Immunogen

    Pure involucrin from human keratinocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab20202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionPart of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
  • Tissue specificityKeratinocytes of epidermis and other stratified squamous epithelia.
  • Sequence similaritiesBelongs to the involucrin family.
  • Post-translational
    modifications
    Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
  • Cellular localizationCytoplasm. Constituent of the scaffolding of the cornified envelope.
  • Information by UniProt
  • Database links
  • Alternative names
    • INVO_HUMAN antibody
    • Involucrin antibody
    • IVL antibody

Anti-Involucrin antibody [SY8] images

  • Ab20202 staining Human skin Staining is localised to the cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-Involucrin antibody [SY8] (ab20202)

ab20202 has not yet been referenced specifically in any publications.

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