Overview

  • Product nameIonotropic Agonists Kit
  • SpecificityA collection of 8 agonists, antagonists and potentiators at AMPA, Kainate and NMDA receptors. Products are freeze-dried to an exact weight so that addition of 1 ml or 0.5 ml of solvent provides a stock solution of 5 mM or 10 mM respectively. The kit contains (S)-AMPA, (ab120005), (S)-5-Fluorowillardiine hydrochloride (ab120399), Cyclothiazide (ab120061), Aniracetam (ab120316), Kainic acid (ab120100), (S)-5-Iodowillardiine hydrochloride (ab120401), NMDA (ab120052) and D-serine (ab120048).
  • Product overview

    A collection of 8 agonists, antagonists and potentiators at AMPA, Kainate and NMDA receptors. Products are freeze-dried to an exact weight so that addition of 1 ml or 0.5 ml of solvent provides a stock solution of 5 mM or 10 mM respectively. The kit contains (S)-AMPA, (ab120005), (S)-5-Fluorowillardiine hydrochloride (ab120399), Cyclothiazide (ab120061), Aniracetam (ab120316), Kainic acid (ab120100), (S)-5-Iodowillardiine hydrochloride (ab120401) , NMDA ab120052) and D-serine (ab120048).

    ReceptorDescription
    AMPA

    (S)-AMPA:


    AMPA agonist.


    Suggested Solvent: Water

    AMPA

    (S)-5-Fluorowillardiine hydrochloride:


    Potent, selective AMPA receptor agonist. Displays higher affinity than AMPA at hGluR1 and hGlurR2, and displays greater selectivity for AMPA receptor subtypes over the kainate receptor hGluR5.


    Suggested Solvent: Water

    AMPA

    Cyclothiazide:


    Positive allosteric modulator of AMPA receptors. Produces a fast inhibition of AMPA receptor desensitization and a much slower potentiation of the AMPA current.


    Suggested Solvent: DMSO

    AMPA

    Aniracetam:


    Nootropic, positive allosteric modulator of AMPA receptors. Slows AMPA receptor deactivation and desensitisation. Antidepressant and anxiolytic in vivo.


    Suggested Solvent: DMSO

    Kainate

    Kainic acid:


    Prototypic agonist at the kainate class of ionotropic glutamate receptors. Potent excitant and neurotoxin, used to model epilepsy and neurodegenerative states.


    Suggested Solvent: Water

    Kainate

    (S)-5-Iodowillardiine hydrochloride:


    Selective GluK1 (formerly GluR5) kainate receptor agonist (EC50 = 83 µM). Partial agonist activity at GluK2 (formerly GluR6/KA2) receptors. Displays low affinity for AMPA and homomeric GluR6 and GluR7 receptors.


    Suggested Solvent: DMSO

    NMDA

    NMDA:


    Excitotoxic amino acid. Prototypic agonist at the ionotropic NMDA glutamate receptor which is involved in long-term potentiation, ischemia, and epilepsy.


    Suggested Solvent: Water

    NMDA

    D-Serine:


    Agonist at the NMDA glycine binding site and the inhibitory post-synaptic glycine receptor.


    Suggested Solvent: Water

     

    A collection of 8 agonists, antagonists and potentiators at AMPA, Kainate and NMDA receptors. Products are freeze-dried to an exact weight so that addition of 1 ml or 0.5 ml of solvent provides a stock solution of 5 mM or 10 mM respectively. The kit contains (S)-AMPA, (ab120048).

  • Notes

    KIT

Properties

  • FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
  • Tissue specificityWidely expressed in brain.
  • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
  • Post-translational
    modifications
    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
  • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
  • Target information above from: UniProt accession P42261 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

References for Ionotropic Agonists Kit (ab120323)

ab120323 has not yet been referenced specifically in any publications.

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These products are freeze dried to an exact weight so adding 0.5 ml in each product will give you conc. of 10 mM.

0.5 ml addition of water or solvent into each product will give 10 mM solution.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"