The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 20674673
Use at an assay dependent concentration. Predicted molecular weight: 96 kDa.
Use at an assay dependent concentration.
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
RelevanceIon channels activated by glutamate are typically divided into two classes. Those sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). AMPAR are comprised of four distinct Glutamate Receptor Subunits (GluR1-4) and they play key roles in almost all excitatory neurotransmission in the brain. The GluR2 subunit is widely expressed throughout the nervous system where it is thought to play key roles in synaptic plasticity and learning and memory.
Ab27225 at a dilution of 1/50, staining ionotropic Glutamate Receptor 2+3 in Human cerebellum by Immunohistochemistry (formalin fixed, paraffin embedded sections).
References for Anti-Ionotropic glutamate receptor 2 + 3 antibody (ab27225)
This product has been referenced in:
Ripka S et al. Glutamate receptor GRIA3--target of CUX1 and mediator of tumor progression in pancreatic cancer. Neoplasia12:659-67 (2010).
Read more (PubMed: 20689760) »
Cabral AL et al. GluR2/3 label expression of the AMPA-type glutamate receptor in the hippocampal formation of the homing pigeon stabilizes just after birth. Neurosci Lett483:73-7 (2010).
Read more (PubMed: 20674673) »