Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)

Overview

  • Product nameAnti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y]
    See all Ionotropic glutamate receptor 2 + 3 primary antibodies
  • Description
    Rabbit monoclonal [EP929Y] to Ionotropic glutamate receptor 2 + 3
  • Specificityab52896 detects both GluR2 + 3 phosphorylated on Serine 880/891 of the mature human, and also unphosphorylated GluR2 + 3.
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Ionotropic glutamate receptor 2 + 3 (phospho S880 + S891). The exact sequence is proprietary.
    (Peptide available as ab205220)

  • Positive control
    • WB: Human brain and fetal tissue lysates and mouse and rat brain tissue lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Ionotropic glutamate receptor 2 + 3 antibody (Biotin) [EP929Y] (ab201534)

Properties

Applications

Our Abpromise guarantee covers the use of ab52896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).Can be blocked with Ionotropic glutamate receptor 2 + 3 peptide (ab205220).
IP 1/100.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • RelevanceIon channels activated by glutamate are typically divided into two classes. Those sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). AMPAR are comprised of four distinct Glutamate Receptor Subunits (GluR1-4) and they play key roles in almost all excitatory neurotransmission in the brain. The GluR2 subunit is widely expressed throughout the nervous system where it is thought to play key roles in synaptic plasticity and learning and memory.
    • Cellular localizationCell Membrane; multi-pass membrane protein.
    • Database links
    • Alternative names
      • AMPA selective glutamate receptor 2 antibody
      • AMPA selective glutamate receptor 3 antibody
      • GluR K2 antibody
      • GluR K3 antibody
      • GLURB antibody
      • GLURC antibody
      • GLUTAMATE RECEPTOR 2 antibody
      • GLUTAMATE RECEPTOR 3 antibody
      • Glutamate receptor ionotropic AMPA 2 antibody
      • Glutamate receptor ionotropic AMPA 3 antibody
      • GRIA2 antibody
      • GRIA3 antibody
      see all

    Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] images

    • All lanes : Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/10000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 98 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/2000 dilution (purified) + Human fetal brain tissue lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 98 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/5000 dilution (unpurified) + Human brain tissue lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 98 kDa
      Observed band size : 98 kDa

    References for Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)

    This product has been referenced in:
    • Zhou JY  et al. Individual and combined effects of rhynchophylline and ketamine on proliferation, NMDAR1 and GluA2/3 protein expression in PC12 cells. Fitoterapia 85:125-9 (2013). ICC/IF ; Rat . Read more (PubMed: 23352746) »
    • Krosnowski K  et al. Diverse populations of intrinsic cholinergic interneurons in the mouse olfactory bulb. Neuroscience 213:161-78 (2012). Read more (PubMed: 22525133) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"