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Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse GluR2 .
(Peptide available as ab25708.)
Our Abpromise guarantee covers the use of ab20673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 3 - 10 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).Can be blocked with Mouse Ionotropic Glutamate receptor 2 peptide (ab25708).|
The band observed at 50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor.
Immunohistochemistical detection of Ionotropic Glutamate receptor 2 using antibody (ab20673) on PFA perfusion-fixed frozen rat brain sections. Primary antibody was used at 1/300, incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary Antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000). The image demonstrates immunostaining obtained in the rat cortex, thin and punctuated cytoplasmic staining is observed as previously described for the hippocampus (J neurosci 2003 Nov 1923(33):10521-30). There is some staining in cellular processes as well (arrows).
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