Overview

  • Product nameAnti-Ionotropic Glutamate receptor 2 antibody
    See all Ionotropic Glutamate receptor 2 primary antibodies
  • Description
    Rabbit polyclonal to Ionotropic Glutamate receptor 2
  • Specificityab52176 detects endogenous levels of total Glutamate receptor 2 protein.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human Glutamate receptor 2 around the phosphorylation site of serine 880 (I-E-S-V-K).

  • Positive control
    • Human brain tissue and extracts from mouse brain.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab52176 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/100. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

Target

  • FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
  • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
  • Post-translational
    modifications
    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
  • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMPA 2 antibody
    • AMPA selective glutamate receptor 2 antibody
    • AMPA-selective glutamate receptor 2 antibody
    • AMPA2 antibody
    • GluA2 antibody
    • GLUR 2 antibody
    • GLUR B antibody
    • GluR K2 antibody
    • GluR-2 antibody
    • GluR-B antibody
    • GluR-K2 antibody
    • GLUR2 antibody
    • GLURB antibody
    • Glutamate receptor 2 antibody
    • Glutamate receptor ionotropic AMPA 2 antibody
    • Glutamate receptor ionotropic antibody
    • Gria2 antibody
    • GRIA2_HUMAN antibody
    • HBGR2 antibody
    see all

Anti-Ionotropic Glutamate receptor 2 antibody images

  • ab52176 at 1/50 dilution staining Ionotropic Glutamate receptor 2 in human brain by Immunohistochemistry, paraffin embedded tissue, in the presence (right image) and absence (left image) of the immunizing peptide.


  • Predicted band size : 98 kDa

References for Anti-Ionotropic Glutamate receptor 2 antibody (ab52176)

This product has been referenced in:
  • Bonnet CS  et al. AMPA/kainate glutamate receptors contribute to inflammation, degeneration and pain related behaviour in inflammatory stages of arthritis. Ann Rheum Dis N/A:N/A (2013). IHC-P ; Rat, Human . Read more (PubMed: 24130267) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"