Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)


  • Product nameAnti-Ionotropic Glutamate receptor 2 antibody [EP966Y]
    See all Ionotropic Glutamate receptor 2 primary antibodies
  • Description
    Rabbit monoclonal [EP966Y] to Ionotropic Glutamate receptor 2
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-Fr or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminus of human GluR2.

  • Positive control
    • Human brain cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab52932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).
IP 1/40.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC/IF,IHC-Fr or IHC-P.
  • Target

    • FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
    • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
    • Post-translational
      Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
    • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
    • Information by UniProt
    • Database links
    • Alternative names
      • AMPA 2 antibody
      • AMPA selective glutamate receptor 2 antibody
      • AMPA-selective glutamate receptor 2 antibody
      • AMPA2 antibody
      • GluA2 antibody
      • GLUR 2 antibody
      • GLUR B antibody
      • GluR K2 antibody
      • GluR-2 antibody
      • GluR-B antibody
      • GluR-K2 antibody
      • GLUR2 antibody
      • GLURB antibody
      • Glutamate receptor 2 antibody
      • Glutamate receptor ionotropic AMPA 2 antibody
      • Glutamate receptor ionotropic antibody
      • Gria2 antibody
      • GRIA2_HUMAN antibody
      • HBGR2 antibody
      see all

    Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] images

    • Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932) at 1/2000 dilution + Human brain cell lysate at 10 µg

      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 99 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932) at 1/500 dilution

      Lane 1 : Human Brain
      Lane 2 : Rat Brain
      Lane 3 : Mouse Brain

      Predicted band size : 99 kDa

      Secondary antibody - anti-rabbit HRP (ab6721)

    References for Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)

    This product has been referenced in:
    • Chen C  et al. Epigenetic modification of PKM? rescues aging-related cognitive impairment. Sci Rep 6:22096 (2016). WB ; Rat . Read more (PubMed: 26926225) »
    • Gascon E  et al. Alterations in microRNA-124 and AMPA receptors contribute to social behavioral deficits in frontotemporal dementia. Nat Med 20:1444-51 (2014). WB, IHC ; Mouse . Read more (PubMed: 25401692) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Non-reduced Non-Denaturing (Native) (stacking gel: 4%, resolving gel: 5-13%)
    Sample Rat Tissue lysate - whole (hippocampi total membrane fraction)
    Specification hippocampi total membrane fraction
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Feb 25 2014

    Thanks for your enquiry. The sequence homology between the immunogen sequence of ab52932 and human Glutamate receptors are as follows. human GluR1: 44.4% human GluR3: 61.5% human GluR4: 56.2% Based on sequence analysis, ab52932 is most like...

    Read More