Anti-Ionotropic Glutamate receptor 2 (phospho S880) antibody (ab52180)

Overview

  • Product name
    Anti-Ionotropic Glutamate receptor 2 (phospho S880) antibody
    See all Ionotropic Glutamate receptor 2 primary antibodies
  • Description
    Rabbit polyclonal to Ionotropic Glutamate receptor 2 (phospho S880)
  • Specificity
    Detects endogenous levels of Glutamate receptor 2 only when phosphorylated at serine 880.
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human Glutamate receptor 2 around the phosphorylation site of serine 880 (I-E-SP-V-K).

  • Positive control
    • Extracts from mouse brain, human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab52180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).

Target

  • Function
    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
  • Sequence similarities
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
  • Post-translational
    modifications
    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
  • Cellular localization
    Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMPA 2 antibody
    • AMPA selective glutamate receptor 2 antibody
    • AMPA-selective glutamate receptor 2 antibody
    • AMPA2 antibody
    • GluA2 antibody
    • GLUR 2 antibody
    • GLUR B antibody
    • GluR K2 antibody
    • GluR-2 antibody
    • GluR-B antibody
    • GluR-K2 antibody
    • GLUR2 antibody
    • GLURB antibody
    • Glutamate receptor 2 antibody
    • Glutamate receptor ionotropic AMPA 2 antibody
    • Glutamate receptor ionotropic antibody
    • Gria2 antibody
    • GRIA2_HUMAN antibody
    • HBGR2 antibody
    see all

Images

  • All lanes : Anti-Ionotropic Glutamate receptor 2 (phospho S880) antibody (ab52180) at 1/500 dilution

    Lane 1 : extracts from mouse brain
    Lane 2 : extracts from mouse brain with blocking phosphopeptide


    Predicted band size : 99 kDa
  • Ab52180 at 1/50 dilution staining Human brain tissue, with and without blocking peptide; paraffin embedded.

References

This product has been referenced in:
  • Bonini D  et al. Acute Footshock Stress Induces Time-Dependent Modifications of AMPA/NMDA Protein Expression and AMPA Phosphorylation. Neural Plast 2016:7267865 (2016). WB . Read more (PubMed: 26966584) »
  • Hao JR  et al. L-Stepholidine rescues memory deficit and synaptic plasticity in models of Alzheimer's disease via activating dopamine D1 receptor/PKA signaling pathway. Cell Death Dis 6:e1965 (2015). WB . Read more (PubMed: 26539912) »

See all 6 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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