Anti-Ionotropic Glutamate receptor 4 antibody (ab115322)

Overview

  • Product name
    Anti-Ionotropic Glutamate receptor 4 antibody
    See all Ionotropic Glutamate receptor 4 primary antibodies
  • Description
    Goat polyclonal to Ionotropic Glutamate receptor 4
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    C-KKLDQREYPGSETP

    , corresponding to internal sequence amino acids 280-293 of Human Ionotropic Glutamate receptor 4 (NP_000820.3; NP_001070711.1; NP_001070712.1).

  • Positive control
    • Human Brain, Cortex; Human Cerebellum lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab115322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 101 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
  • Sequence similarities
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA4 subfamily.
  • Post-translational
    modifications
    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
  • Cellular localization
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMPA 4 antibody
    • AMPA selective glutamate receptor 4 antibody
    • AMPA-selective glutamate receptor 4 antibody
    • AMPA4 antibody
    • GluA 4 antibody
    • GluA4 antibody
    • GluR 4 antibody
    • GluR D antibody
    • GluR-4 antibody
    • GluR-D antibody
    • GluR4 antibody
    • GLUR4C antibody
    • GLURD antibody
    • Glutamate receptor 4 antibody
    • Glutamate receptor ionotrophic AMPA 4 antibody
    • Glutamate receptor ionotropic antibody
    • GRIA 4 antibody
    • Gria4 antibody
    • GRIA4_HUMAN antibody
    see all

Images

  • Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ab115322 at 5 µg/ml, followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-Ionotropic Glutamate receptor 4 antibody (ab115322) at 0.3 µg/ml (incubated for 1 hour) + Human Cerebellum lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 101 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References

ab115322 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab115322.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up