Overview

  • Product name
    Anti-Ionotropic Glutamate receptor 4 antibody
    See all Ionotropic Glutamate receptor 4 primary antibodies
  • Description
    Goat polyclonal to Ionotropic Glutamate receptor 4
  • Specificity
    ab77407 is expected to recognise all three reported isoforms (NP_000820.3; NP_001070711.1; NP_001070712.1).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    C-KKLDQREYPGSETP

    , corresponding to internal sequence amino acids 280-293 of Human Ionotropic Glutamate receptor 4 (NP_000820.3; NP_001070711.1; NP_001070712.1).

  • Positive control
    • Human brain (cerebellum) lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/128000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 101 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
    • Sequence similarities
      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA4 subfamily.
    • Post-translational
      modifications
      Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
    • Cellular localization
      Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AMPA 4 antibody
      • AMPA selective glutamate receptor 4 antibody
      • AMPA-selective glutamate receptor 4 antibody
      • AMPA4 antibody
      • GluA 4 antibody
      • GluA4 antibody
      • GluR 4 antibody
      • GluR D antibody
      • GluR-4 antibody
      • GluR-D antibody
      • GluR4 antibody
      • GLUR4C antibody
      • GLURD antibody
      • Glutamate receptor 4 antibody
      • Glutamate receptor ionotrophic AMPA 4 antibody
      • Glutamate receptor ionotropic antibody
      • GRIA 4 antibody
      • Gria4 antibody
      • GRIA4_HUMAN antibody
      see all

    Images

    • Anti-Ionotropic Glutamate receptor 4 antibody (ab77407) at 0.3 µg/ml + human cerebellum lysate in RIPA buffer at 35 µg

      Predicted band size : 101 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Gascon E  et al. Alterations in microRNA-124 and AMPA receptors contribute to social behavioral deficits in frontotemporal dementia. Nat Med 20:1444-51 (2014). WB, IHC ; Mouse . Read more (PubMed: 25401692) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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