The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: Antibody detection limit dilution 1/128000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 101 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA4 subfamily.
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
Gascon E et al. Alterations in microRNA-124 and AMPA receptors contribute to social behavioral deficits in frontotemporal dementia. Nat Med20:1444-51 (2014).
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