RabMAb

Anti-Ionotropic Glutamate receptor 4 antibody [EPR2512(2)] (ab109431)

Overview

  • Product nameAnti-Ionotropic Glutamate receptor 4 antibody [EPR2512(2)]See all Ionotropic Glutamate receptor 4 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR2512(2)] to Ionotropic Glutamate receptor 4
  • Tested applicationsWB, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the intracellular domain in Human Ionotropic Glutamate receptor 4.

  • Positive control
    • Rat brain, Mouse brain, Human cerebellum, and SH-SY5Y lysates; SH-SY5Y cells
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab109431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 101 kDa.
Flow Cyt 1/10 - 1/100.
  • Application notesIs unsuitable for ICC,IHC-P or IP.
  • Target

    • FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
    • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA4 subfamily.
    • Post-translational
      modifications
      Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
    • Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
    • Target information above from: UniProt accession P48058 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • AMPA 4 antibody
      • AMPA selective glutamate receptor 4 antibody
      • AMPA-selective glutamate receptor 4 antibody
      • AMPA4 antibody
      • GluA 4 antibody
      • GluA4 antibody
      • GluR 4 antibody
      • GluR D antibody
      • GluR-4 antibody
      • GluR-D antibody
      • GluR4 antibody
      • GLUR4C antibody
      • GLURD antibody
      • Glutamate receptor 4 antibody
      • Glutamate receptor ionotrophic AMPA 4 antibody
      • Glutamate receptor ionotropic antibody
      • GRIA 4 antibody
      • Gria4 antibody
      • GRIA4_HUMAN antibody
      see all

    Anti-Ionotropic Glutamate receptor 4 antibody [EPR2512(2)] images

    • All lanes : Anti-Ionotropic Glutamate receptor 4 antibody [EPR2512(2)] (ab109431) at 1/1000 dilution

      Lane 1 : Rat brain lysate
      Lane 2 : Mouse brain lysate
      Lane 3 : Human cerebellum lysate
      Lane 4 : SH-SY5Y lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 101 kDa
    • Flow cytometric analysis of permeabilized SH-SY5Y cells using ab109431 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References for Anti-Ionotropic Glutamate receptor 4 antibody [EPR2512(2)] (ab109431)

    ab109431 has not yet been referenced specifically in any publications.

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