Anti-IP10 antibody (ab9807)
Key features and details
- Rabbit polyclonal to IP10
- Suitable for: WB, Sandwich ELISA
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-IP10 antibody
See all IP10 primary antibodies -
Description
Rabbit polyclonal to IP10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Sandwich ELISAmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human IP10. Highly pure (>98%) recombinant hIP-10 (human Interferon Inducible Protein 10)
Database link: P02778 -
Positive control
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General notes
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Sandwich ELISA |
Use at an assay dependent concentration.
To detect hIP-10 by direct ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with biotinylated anti-IP10 antibody (ab271283), allows the detection of 0.2 - 0.4 ng/well of recombinant hIP-10. |
Notes |
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WB
Use at an assay dependent concentration. To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Sandwich ELISA
Use at an assay dependent concentration. To detect hIP-10 by direct ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with biotinylated anti-IP10 antibody (ab271283), allows the detection of 0.2 - 0.4 ng/well of recombinant hIP-10. |
Target
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Function
Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsCXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3627 Human
- Entrez Gene: 245920 Rat
- Omim: 147310 Human
- SwissProt: P02778 Human
- SwissProt: P48973 Rat
- Unigene: 632586 Human
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Alternative names
- Interferon gamma induced factor MOB1, mouse, homolog of antibody
- Interferon gamma induced protein 10 antibody
- 10 kDa interferon gamma induced protein antibody
see all
Images
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ab9807 detecting IP-10 by sandwich ELISA.
Datasheets and documents
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Datasheet download
References (33)
ab9807 has been referenced in 33 publications.
- Braegelmann C et al. Epigallocatechin-3-gallate exhibits anti-inflammatory effects in a human interface dermatitis model-implications for therapy. J Eur Acad Dermatol Venereol 36:144-153 (2022). PubMed: 34585800
- Park TI et al. Routine culture and study of adult human brain cells from neurosurgical specimens. Nat Protoc 17:190-221 (2022). PubMed: 35022619
- Kim M et al. Role of CXCL10 in the progression of in situ to invasive carcinoma of the breast. Sci Rep 11:18007 (2021). PubMed: 34504204
- El-Domyati M et al. Systemic CXCL10 is a predictive biomarker of vitiligo lesional skin infiltration, PUVA, NB-UVB and corticosteroid treatment response and outcome. Arch Dermatol Res N/A:N/A (2021). PubMed: 33866437
- Liu Q et al. Pathological changes in the lungs and lymphatic organs of 12 COVID-19 autopsy cases. Natl Sci Rev 7:1868-1878 (2020). PubMed: 34676085