Overview

  • Product name
    Anti-IP3 receptor antibody
    See all IP3 receptor primary antibodies
  • Description
    Rabbit polyclonal to IP3 receptor
  • Tested applications
    Suitable for: IP, WB, ICC/IF, ICC, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Dog, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human IP3 receptor aa 1829-1848.
    Sequence:

    NKKKDDEVDRDAPSRKKAKE


    (Peptide available as ab5839)

Properties

Applications

Our Abpromise guarantee covers the use of ab5804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 314 kDa.Can be blocked with IP3 receptor peptide (ab5839).
ICC/IF Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-Fr 1/250.

Staining of IP3R-I in rat cerebellum with ab5804 results in staining of Purkinje cells.

IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate.
  • Tissue specificity
    Widely expressed.
  • Involvement in disease
    Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory.
  • Sequence similarities
    Belongs to the InsP3 receptor family.
    Contains 5 MIR domains.
  • Domain
    The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.
  • Post-translational
    modifications
    Phosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels.
    Phosphorylated on tyrosine residues.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4 antibody
    • 5-trisphosphate receptor antibody
    • 5-trisphosphate receptor type 1 antibody
    • DKFZp313E1334 antibody
    • DKFZp313N1434 antibody
    • inositol 1 4 5 triphosphate receptor type 1 antibody
    • Inositol 1 4 5 trisphosphate Receptor Type 1 antibody
    • Inositol 1 antibody
    • InsP3R1 antibody
    • IP3 antibody
    • IP3 receptor antibody
    • IP3 receptor isoform 1 antibody
    • IP3R 1 antibody
    • IP3R antibody
    • IP3R1 antibody
    • ITPR 1 antibody
    • Itpr1 antibody
    • ITPR1_HUMAN antibody
    • SCA15 antibody
    • SCA16 antibody
    • SCA29 antibody
    • Type 1 inositol 1 4 5 trisphosphate receptor antibody
    • Type 1 inositol 1 antibody
    • Type 1 InsP3 receptor antibody
    see all

Anti-IP3 receptor antibody images

  • Shows immunolocalization of ITPR1 in rat cerebellum using ab5804.

  • IHC image of ab5804 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5804, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-IP3 receptor antibody (ab5804)

This product has been referenced in:
  • Halbach MV  et al. Atxn2 Knockout and CAG42-Knock-in Cerebellum Shows Similarly Dysregulated Expression in Calcium Homeostasis Pathway. Cerebellum 16:68-81 (2017). WB ; Mouse . Read more (PubMed: 26868665) »
  • Barcelona PF  et al. p75NTR and Its Ligand ProNGF Activate Paracrine Mechanisms Etiological to the Vascular, Inflammatory, and Neurodegenerative Pathologies of Diabetic Retinopathy. J Neurosci 36:8826-41 (2016). Read more (PubMed: 27559166) »

See all 9 Publications for this product

Product Wall

I would recommend to follow the protocol we have on our website. The use of 2 times the concentration of peptide (compared to the antibody concentration) represents a large and sufficient excess to block the antibody. I hope this is helpful. Do not hes...

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Thank you for confirming these details and for your cooperation. As agreed, I have issued a free of charge replacement with the order number ******. To check the status of the order please contact our Customer Service team and reference this number...

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Thank you for confirming these details and for your cooperation. I can confirm that : - the blocking peptide for ab5804 is ab5839 - the immunogen of ab103278 is aa 1155-1184 of the isoform A (SwissProt: Q9NQ66-1) Because we carry over 70,000 ...

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Thank you for contacting us and for sending images of the results obtained with ab5804 and ab77743. 1)  ab5804 : I looked at the image of the blot and I am positive that the band observed above the 250kDa marker is ITPR1. As explained in my previou...

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Thank you very much for your inquiry and your interest in our antibodies. Indeed, we do not have ITPR1 antibodies tested against bovine in our catalogue currently. We are however able to provide you with a testing offer, please see below. ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - other (DO11.10 Thymocyte hybridoma)
Total protein in input
25 µg
Specification
DO11.10 Thymocyte hybridoma
Immuno-precipitation step
Protein A/G
Username

Dr. Jonathan Rud

Verified customer

Submitted Dec 28 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (DO11.10 T-cell Hybridoma)
Loading amount
25 µg
Specification
DO11.10 T-cell Hybridoma
Gel Running Conditions
Reduced Denaturing (4% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Jonathan Rud

Verified customer

Submitted Dec 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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