Synthetic peptide conjugated to KLH derived from within residues 2700 to the C-terminus of Human ITPR1.
Our Abpromise guarantee covers the use of ab97823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 314 kDa (predicted molecular weight: 314 kDa).|
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: IP3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: SHSY-5Y whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab97823 observed at 314 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab97823 was shown to specifically recognize IP3 receptor in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when IP3 knockout cells were examined. Wild-type and IP3 receptor knockout samples were subjected to SDS-PAGE. Ab97823 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1 µg/ml and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
ab97823 has not yet been referenced specifically in any publications.