Anti-IP3 receptor antibody [EPR4537] (ab108517)


  • Product nameAnti-IP3 receptor antibody [EPR4537]
    See all IP3 receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR4537] to IP3 receptor
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human ITPR1.

  • Positive control
    • Rat brain, SH-SY5Y, Mouse brain, and HeLa lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab108517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 314 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionIntracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate.
    • Tissue specificityWidely expressed.
    • Involvement in diseaseDefects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory.
    • Sequence similaritiesBelongs to the InsP3 receptor family.
      Contains 5 MIR domains.
    • DomainThe receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.
    • Post-translational
      Phosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels.
      Phosphorylated on tyrosine residues.
    • Cellular localizationEndoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4 antibody
      • 5-trisphosphate receptor antibody
      • 5-trisphosphate receptor type 1 antibody
      • DKFZp313E1334 antibody
      • DKFZp313N1434 antibody
      • inositol 1 4 5 triphosphate receptor type 1 antibody
      • Inositol 1 4 5 trisphosphate Receptor Type 1 antibody
      • Inositol 1 antibody
      • InsP3R1 antibody
      • IP3 antibody
      • IP3 receptor antibody
      • IP3 receptor isoform 1 antibody
      • IP3R 1 antibody
      • IP3R antibody
      • IP3R1 antibody
      • ITPR 1 antibody
      • Itpr1 antibody
      • ITPR1_HUMAN antibody
      • SCA15 antibody
      • SCA16 antibody
      • SCA29 antibody
      • Type 1 inositol 1 4 5 trisphosphate receptor antibody
      • Type 1 inositol 1 antibody
      • Type 1 InsP3 receptor antibody
      see all

    Anti-IP3 receptor antibody [EPR4537] images

    • Predicted band size : 314 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: IP3 receptor knockout HAP1 cell lysate (20 µg)
      Lane 3: Human brain tissue lysate (20 µg)
      Lane 4: SH-SY5Y cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab108517 observed at 270 kDa. Red - loading control, ab18058, observed at 124 kDa.
      ab108517 was shown to recognize IP3 receptor when IP3 receptor knockout samples were used, along with additional cross-reactive bands. Wild-type and IP3 receptor knockout samples were subjected to SDS-PAGE. ab108517 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-IP3 receptor antibody [EPR4537] (ab108517) at 1/1000 dilution

      Lane 1 : Rat brain lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : Mouse brain lysate
      Lane 4 : HeLa brain lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 314 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-IP3 receptor antibody [EPR4537] (ab108517)

    ab108517 has not yet been referenced specifically in any publications.

    Product Wall

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