Anti-ITPKC antibody (ab188007)
Key features and details
- Rabbit polyclonal to ITPKC
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ITPKC antibody -
Description
Rabbit polyclonal to ITPKC -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ITPKC aa 250-400.
Database link: Q96DU7 -
Positive control
- IHC-P:Human testis tissue WB: RT4 and U-251 MG sp cell lysates. ICC/IF: U-2OS cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 75 kDa.
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 75 kDa. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Function
Can phosphorylate inositol 2,4,5-triphosphate to inositol 2,4,5,6-tetraphosphate. -
Tissue specificity
Highly expressed in pancreas, skeletal muscle, liver, placenta and weakly in kidney and brain. -
Involvement in disease
Defects in ITPKC are a cause of Kawasaki disease (KWD) [MIM:611775]; also known as mucocutaneous lymph node syndrome or infantile polyarteritis. Kawasaki disease is an acute, self-limited vasculitis of infants and children characterized by prolonged fever unresponsive to antibiotics, polymorphous skin rash, erythema of the oral mucosa, lips, and tongue, erythema of the palms and soles, bilateral conjunctival injection, and cervical lymphadenopathy. Coronary artery aneurysms develop in 15 to 25% of those left untreated, making Kawasaki disease the leading cause of acquired heart disease among children in developed countries. -
Sequence similarities
Belongs to the inositol phosphokinase (IPK) family. -
Cellular localization
Nucleus. Cytoplasm. Shuttles actively between nucleus and cytoplasm with both nuclear import and nuclear export activity. - Information by UniProt
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Database links
- Entrez Gene: 80271 Human
- Omim: 606476 Human
- SwissProt: Q96DU7 Human
- Unigene: 515415 Human
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Alternative names
- 4 antibody
- 5-trisphosphate 3-kinase C antibody
- Inositol 1 antibody
see all
Images
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All lanes : Anti-ITPKC antibody (ab188007) at 1/500 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251MG sp cell lysate
Predicted band size: 75 kDa -
Immunocytochemistry/immunofluorescent analysis of U-2OS cells staining ITPKC antibody with ab18807 at 1μg/ml (green).
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Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ITPKC with ab188007 at 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab188007 has not yet been referenced specifically in any publications.