The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Is unsuitable for ICC/IF.
May play a part in organizing microtubules in relation to the function of the centrosome/spindle pole during mitosis. May also contribute to the regulation of chromosome segregation and cytokinesis.
Isoform 1 and isoform 2 are expressed in testis. Elevated expression levels were seen only in a subset of cancer cells such as HepG2, HuH7 and HeLa cells. Expression is maximum at M phase.
Involvement in disease
Defects in AURKC are the cause of male infertility with large-headed multiflagellar polyploid spermatozoa (MIMPS) [MIM:243060]; also known as infertility associated with multi-tailed spermatozoa and excessive DNA.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily. Contains 1 protein kinase domain.
Chromosome. Cytoplasm > cytoskeleton > spindle. Distributes in the condensed chromosomes during prophase to metaphase. After entering anaphase, there is a dissociation from separated chromosomes and a redistribution to midzone microtubules, and finally remaines in the midbody during cytokinesis.
Western blot - Anti-IPL-1/STK13/Aurora C antibody (ab38297)This image is courtesy of Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).
All lanes : Anti-IPL-1/STK13/Aurora C antibody (ab38297)
Lane 1 : 293 cell lysate expressing Flag tag Lane 2 : 293 cell lysate expressing Flag tagged Aurora A Lane 3 : 293 cell lysate expressing Flag tagged Aurora B Lane 4 : 293 cell lysate expressing Flag tagged Aurora C
Predicted band size : 36 kDa Observed band size : 36 kDa Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
This image is courtesy of Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).
Western blot - Anti-IPL-1/STK13/Aurora C antibody (ab38297)
Anti-IPL-1/STK13/Aurora C antibody (ab38297) + 293 cell lysate at 35 µg