Recombinant
RabMAb

Anti-IPL-1/STK13/Aurora C antibody [EP1011Y] (ab46783)

Overview

  • Product name
    Anti-IPL-1/STK13/Aurora C antibody [EP1011Y]
    See all IPL-1/STK13/Aurora C primary antibodies
  • Description
    Rabbit monoclonal [EP1011Y] to IPL-1/STK13/Aurora C
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human Aurora C.

  • Positive control
    • HepG2 cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP1011Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab46783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

    This antibody does not seem to be suitable for ICC/IF (please see abreview).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May play a part in organizing microtubules in relation to the function of the centrosome/spindle pole during mitosis. May also contribute to the regulation of chromosome segregation and cytokinesis.
    • Tissue specificity
      Isoform 1 and isoform 2 are expressed in testis. Elevated expression levels were seen only in a subset of cancer cells such as HepG2, HuH7 and HeLa cells. Expression is maximum at M phase.
    • Involvement in disease
      Defects in AURKC are the cause of male infertility with large-headed multiflagellar polyploid spermatozoa (MIMPS) [MIM:243060]; also known as infertility associated with multi-tailed spermatozoa and excessive DNA.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
      Contains 1 protein kinase domain.
    • Cellular localization
      Chromosome. Cytoplasm > cytoskeleton > spindle. Distributes in the condensed chromosomes during prophase to metaphase. After entering anaphase, there is a dissociation from separated chromosomes and a redistribution to midzone microtubules, and finally remaines in the midbody during cytokinesis.
    • Information by UniProt
    • Database links
    • Alternative names
      • AURKC antibody
      • AIE 2 antibody
      • Aie1 antibody
      • AIE2 antibody
      • AIK 3 antibody
      • AIK3 antibody
      • ARK 3 antibody
      • ARK-3 antibody
      • ARK3 antibody
      • Aur C antibody
      • AurC antibody
      • Aurkc antibody
      • AURKC_HUMAN antibody
      • aurora 3 antibody
      • Aurora C antibody
      • Aurora kinase C antibody
      • aurora related kinase 3 antibody
      • Aurora-related kinase 3 antibody
      • Aurora/Ipl1 related kinase 3 antibody
      • Aurora/Ipl1-related kinase 3 antibody
      • Aurora/Ipl1/Eg2 protein 2 antibody
      • EC 2.7.11.1 antibody
      • Serine threonine protein kinase 13 antibody
      • serine/threonine kinase 13 (aurora/IPL1 like) antibody
      • serine/threonine protein kinase aurora C antibody
      • Serine/threonine-protein kinase 13 antibody
      • Serine/threonine-protein kinase aurora-C antibody
      • SPGF5 antibody
      • STK13 antibody
      see all

    Images

    • Anti-IPL-1/STK13/Aurora C antibody [EP1011Y] (ab46783) at 1/1000 dilution + HepG2 lysate at 10 µg

      Secondary
      goat anti rabbit HRP at 2000

      Predicted band size : 36 kDa
      Observed band size : 36 kDa

    References

    ab46783 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jul 02 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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