Overview

  • Product name
  • Description
    Rabbit polyclonal to IPMK
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 26 and 265 of Human IPMK (NP_689416).

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2 and Raji cell lysates for WB and HeLa cells for IF.

Properties

Applications

Our Abpromise guarantee covers the use of ab96753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
ICC/IF 1/100 - 1/200.

Target

Images

  • Anti-IPMK antibody (ab96753) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 47 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96753 at 1/200 dilution. Lower image merged with DNA probe.

References

ab96753 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (FAO rat hepatoma cells)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
25 µg
Treatment
100 nM insulin for 5 minutes
Specification
FAO rat hepatoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Sep 29 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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