Overview

  • Product name
    Anti-IPO 38 antibody [IPO-38]
    See all IPO 38 primary antibodies
  • Description
    Mouse monoclonal [IPO-38] to IPO 38
  • Tested applications
    Suitable for: ELISA, IHC-P, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A BALB/c mouse was immunized with spleen cells of a human patient with hairy cell leukemia.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 2.92% Sodium chloride
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    This protein A purified mouse monoclonal antibody reacts with a 14 -16 kDa protein as demonstrated by western blot analysis on the Raji cell line. This antigen was detected after 12 h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes. Cross reactivity is likely to occur with mouse and rat. Reactivity with other sources has not been determined.
  • Clonality
    Monoclonal
  • Clone number
    IPO-38
  • Isotype
    IgM
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
IHC-Fr Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
WB 1/50 - 1/100.

Target

  • Relevance
    IPO-38 antigen is present in the nuclei of proliferating cells such as Hodgkin's disease and non-Hodgkin's lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes. IPO-38 can be a useful marker of cell proliferation during monitoring of tumor progression.

References

This product has been referenced in:
  • Mikhalap SV  et al. Monoclonal antibody IPO-38 recognizes a novel nuclear antigen of proliferating cells. In Leucocyte Typing VI edited by Kishimoto T et al. Garland Publishing, Inc. New York & London. :609-610 (1997). Read more (PubMed: ) »
  • Sidorenko SP  et al. [Monoclonal antibodies of the IPO series in studying and diagnosing malignant lymphoproliferative diseases] Gematol Transfuziol 35:19-22 (1990). Read more (PubMed: 2373343) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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