Overview

  • Product nameAnti-IPP antibody
    See all IPP primary antibodies
  • Description
    Rabbit polyclonal to IPP
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 432-481 (CCEMQGLIYV IGGISNEGIE LRSFEVYDPL SKRWSPLPPM GTRRAYLGVA) of Human IPP (NP_005888).

  • Positive control
    • Hela cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab81692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

Target

  • RelevanceIntracisternal A particle promoted polypeptide (IPP) is a member of the kelch family of proteins, characterized by a 50 amino acid repeat which interacts with actin. The kelch domain of IPP consists of six C terminal tandem arranged repeats. IPP may play a role in organizing the actin cytoskeleton. IPP also contains an N terminal POZ protein protein interaction domain. The POZ domain (also called BTB domain) is present near the N terminus of a fraction of zinc finger proteins and in proteins that contain the pfam01344 motif such as kelch and pox virus proteins. The BTB/POZ domain mediates homomeric dimerization and in some instances heteromeric dimerization. POZ domains from several zinc finger proteins have been shown to mediate transcriptional repression and to interact with components of histone deacetylase co repressor complexes including N-coR and SMRT.
  • Cellular localizationCytoplasm, cytoskeleton.
  • Database links
  • Alternative names
    • Actin binding protein IPP antibody
    • Intracisternal A particle promoted polypeptide antibody
    • IPP POZ antibody
    • Kelch like protein 27 antibody
    • KLHL27 antibody
    • MIPP protein antibody
    see all

Anti-IPP antibody images

  • Anti-IPP antibody (ab81692) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 39 kDa,45 kDa. We are unsure as to the identity of these extra bands.

References for Anti-IPP antibody (ab81692)

ab81692 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"