Recombinant
RabMAb

Anti-IPP antibody [EPR15575] (ab192266)

Overview

  • Product name
    Anti-IPP antibody [EPR15575]
  • Description
    Rabbit monoclonal [EPR15575] to IPP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human IPP aa 200-350. The exact sequence is proprietary.
    Database link: Q9Y573

  • Positive control
    • HepG2, Jurkat and Human testis lysates: mouse brain heart and kidney lysates: Rat heart and kidney lysates: Human placenta and Mouse kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR15575
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/2000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

Target

  • Relevance
    Intracisternal A particle promoted polypeptide (IPP) is a member of the kelch family of proteins, characterized by a 50 amino acid repeat which interacts with actin. The kelch domain of IPP consists of six C terminal tandem arranged repeats. IPP may play a role in organizing the actin cytoskeleton. IPP also contains an N terminal POZ protein protein interaction domain. The POZ domain (also called BTB domain) is present near the N terminus of a fraction of zinc finger proteins and in proteins that contain the pfam01344 motif such as kelch and pox virus proteins. The BTB/POZ domain mediates homomeric dimerization and in some instances heteromeric dimerization. POZ domains from several zinc finger proteins have been shown to mediate transcriptional repression and to interact with components of histone deacetylase co repressor complexes including N-coR and SMRT.
  • Cellular localization
    Cytoplasm, cytoskeleton.
  • Database links
  • Alternative names
    • Actin binding protein IPP antibody
    • Intracisternal A particle promoted polypeptide antibody
    • IPP POZ antibody
    • Kelch like protein 27 antibody
    • KLHL27 antibody
    • MIPP protein antibody
    see all

Images

  • All lanes : Anti-IPP antibody [EPR15575] (ab192266) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

  • All lanes : Anti-IPP antibody [EPR15575] (ab192266) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Rat heart lysate
    Lane 5 : Rat kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 65 kDa
    Observed band size: 65 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling IPP with ab192266 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.

  • Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling IPP with ab192266 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.

References

ab192266 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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