The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionPhosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). InsP6 is involved in many processes such as mRNA export, non-homologous end-joining, endocytosis, ion channel regulation. It also protects cells from TNF-alpha-induced apoptosis.
Tissue specificityUbiquitously expressed, with high expression in heart, brain, testis and placenta.
Sequence similaritiesBelongs to the IPK1 type 2 family.
DomainThe EXKPK motif is conserved in inositol-pentakisphosphate 2-kinases of both family 1 and 2.