Overview

  • Product nameAnti-IQGAP1 antibody [0.T.72]
    See all IQGAP1 primary antibodies
  • Description
    Mouse monoclonal [0.T.72] to IQGAP1
  • SpecificityRecognizes human IQGAP1 at 185kD. (Does not recognize IQGAP2).
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment by a Glutaraldehyde linker, corresponding to C terminal amino acids 863-1657 of Human IQGAP1.

  • Positive control
    • HepG2 RIPA cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab33542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at a concentration of 4 µg/ml (immunoprecipitated IQGAP1 from 500µg of a HepG2 RIPA lysate).
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 184 kDa. A goat anti-mouse secondary antibody conjugated to HRP can be used as secondary antibody together with a chemiluminescence detection system.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.
    • Tissue specificityExpressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.
    • Sequence similaritiesContains 1 CH (calponin-homology) domain.
      Contains 4 IQ domains.
      Contains 1 Ras-GAP domain.
      Contains 1 WW domain.
    • DomainRegions C1 and C2 can either interact with nucleotide-free CDC42, or interact together, depending on the phosphorylation state of Ser-1443. When Ser-1443 is not phosphorylated, C1 and C2 interact, which prevents binding of nucleotide-free CDC42 and promotes binding of GTP-bound CDC42. Phosphorylation of Ser-1443 prevents interaction between C1 and C2, which opens the structure of the C-terminus and allows binding and sequestration of nucleotide-free CDC42 on both C1 and C2.
    • Post-translational
      modifications
      Phosphorylation of Ser-1443 by PKC prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • HUMORFA01 antibody
      • IQ motif containing GTPase activating protein 1 antibody
      • IQGA1_HUMAN antibody
      • Iqgap1 antibody
      • KIAA0051 antibody
      • p195 antibody
      • Ras GTPase activating like protein 1 antibody
      • Ras GTPase-activating-like protein IQGAP1 antibody
      • RasGAP-like with IQ motifs antibody
      • SAR1 antibody
      see all

    References for Anti-IQGAP1 antibody [0.T.72] (ab33542)

    This product has been referenced in:
    • Liu C  et al. IQGAP1 suppresses TßRII-mediated myofibroblastic activation and metastatic growth in liver. J Clin Invest 123:1138-56 (2013). Human . Read more (PubMed: 23454766) »
    • Liang S  et al. Honokiol inhibits HepG2 migration via down-regulation of IQGAP1 expression discovered by a quantitative pharmaceutical proteomic analysis. Proteomics 10:1474-83 (2010). WB ; Human . Read more (PubMed: 20127691) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"