Overview

  • Product nameAnti-IQGAP1 antibody
    See all IQGAP1 primary antibodies
  • Description
    Rabbit polyclonal to IQGAP1
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 1 and 50 of human IQGAP1 (NP_003861.1)

  • Positive control
    • HeLa and 293T whole cell lysates. This antibody gave a positive result in IF in the following Formaldehyde/Methanol fixed cell line: SKNSH.

Properties

Applications

Our Abpromise guarantee covers the use of ab86064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000 - 1/10000. Detects a band of approximately 189 kDa (predicted molecular weight: 189 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/100.

Target

  • FunctionBinds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.
  • Tissue specificityExpressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.
  • Sequence similaritiesContains 1 CH (calponin-homology) domain.
    Contains 4 IQ domains.
    Contains 1 Ras-GAP domain.
    Contains 1 WW domain.
  • DomainRegions C1 and C2 can either interact with nucleotide-free CDC42, or interact together, depending on the phosphorylation state of Ser-1443. When Ser-1443 is not phosphorylated, C1 and C2 interact, which prevents binding of nucleotide-free CDC42 and promotes binding of GTP-bound CDC42. Phosphorylation of Ser-1443 prevents interaction between C1 and C2, which opens the structure of the C-terminus and allows binding and sequestration of nucleotide-free CDC42 on both C1 and C2.
  • Post-translational
    modifications
    Phosphorylation of Ser-1443 by PKC prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • HUMORFA01 antibody
    • IQ motif containing GTPase activating protein 1 antibody
    • IQGA1_HUMAN antibody
    • Iqgap1 antibody
    • KIAA0051 antibody
    • p195 antibody
    • Ras GTPase activating like protein 1 antibody
    • Ras GTPase-activating-like protein IQGAP1 antibody
    • RasGAP-like with IQ motifs antibody
    • SAR1 antibody
    see all

Anti-IQGAP1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling IQGAP1 with ab86064 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB and a DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100).
  • All lanes : Anti-IQGAP1 antibody (ab86064) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 189 kDa
    Observed band size : 189 kDa
    Additional bands at : 238 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds
  • Detection of IQGAP1 by Western Blot of Immunprecipitate.
    ab86064 at 0.4µg/ml staining IQGAP1 in HeLa whole cell lysate immunoprecipitated using ab86064 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.
  • ab86064 staining IQGAP1 in human pacreatic islets by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate. Samples were then blocked with 5% serum for 1 hour at 20°C and then incubated with ab86064 at a 1/100 dilution for 8 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.

    See Abreview

  • ab86064 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86064 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol fixed (100%, 5min) SKNSH cells at 1ug/ml.

References for Anti-IQGAP1 antibody (ab86064)

This product has been referenced in:
  • Jameson KL  et al. IQGAP1 scaffold-kinase interaction blockade selectively targets RAS-MAP kinase-driven tumors. Nat Med 19:626-30 (2013). WB ; Human . Read more (PubMed: 23603816) »
  • Tekletsadik YK  et al. A conserved role of IQGAP1 in regulating TOR complex 1. J Cell Sci 125:2041-52 (2012). ICC/IF ; Human . Read more (PubMed: 22328503) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa Cells)
Permeabilization Yes - 1% Triton X-100 in PBS
Specification HeLa Cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

Submitted Apr 08 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (Cos7 green monkey kidney fibroblast like)
Permeabilization Yes - 0.1% triton
Specification Cos7 green monkey kidney fibroblast like
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Paraformaldehyde
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Mr. Stephane Gross

Verified customer

Submitted May 13 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (pancreastic islets)
Loading amount 25 µg
Specification pancreastic islets
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
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Submitted Apr 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (lung)
Specification lung
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Apr 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (pancreatic islets)
Specification pancreatic islets
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"