Overview

  • Product name
  • Description
    Rabbit polyclonal to IQGAP1
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region within N-terminal amino acids 1 - 50 of Human IQGAP1 (NP_003861.1)

  • Positive control
    • Breast, colon, ovarian or prostate carcinoma; testicular seminoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab87262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500. Antigen retrieval with citrate or Tris-EDTA buffer pH 9.0 may enhance staining.
    Likely to work with frozen sections.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.
    • Tissue specificity
      Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.
    • Sequence similarities
      Contains 1 CH (calponin-homology) domain.
      Contains 4 IQ domains.
      Contains 1 Ras-GAP domain.
      Contains 1 WW domain.
    • Domain
      Regions C1 and C2 can either interact with nucleotide-free CDC42, or interact together, depending on the phosphorylation state of Ser-1443. When Ser-1443 is not phosphorylated, C1 and C2 interact, which prevents binding of nucleotide-free CDC42 and promotes binding of GTP-bound CDC42. Phosphorylation of Ser-1443 prevents interaction between C1 and C2, which opens the structure of the C-terminus and allows binding and sequestration of nucleotide-free CDC42 on both C1 and C2.
    • Post-translational
      modifications
      Phosphorylation of Ser-1443 by PKC prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • HUMORFA01 antibody
      • IQ motif containing GTPase activating protein 1 antibody
      • IQGA1_HUMAN antibody
      • Iqgap1 antibody
      • KIAA0051 antibody
      • p195 antibody
      • Ras GTPase activating like protein 1 antibody
      • Ras GTPase-activating-like protein IQGAP1 antibody
      • RasGAP-like with IQ motifs antibody
      • SAR1 antibody
      see all

    Images

    • ab87262 at a 1/100 dilution staining IQGAP1 in Human breast carcinoma tissue.
      Detection: Goat anti-Rabbit IgG conjugated with a red-fluorescent dye, at a 1/100 dilution.
    • ab87262 at a 1/100 dilution staining IQGAP1 in Human breast carcinoma tissue. DAB staining.

    References

    ab87262 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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