Recombinant
RabMAb

Anti-IQGAP2 antibody [EPR13382] (ab181127)

Overview

  • Product name
    Anti-IQGAP2 antibody [EPR13382]
    See all IQGAP2 primary antibodies
  • Description
    Rabbit monoclonal [EPR13382] to IQGAP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human IQGAP2 aa 1300-1500. The exact sequence is proprietary.
    Database link: Q13576

  • Positive control
    • HepG2 or Human fetal liver lysate, Human colonic carcinoma, liver or skeletal muscle tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab181127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 181 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin.
  • Tissue specificity
    Isoform 2 expression is enhanced in testis.
  • Sequence similarities
    Contains 1 CH (calponin-homology) domain.
    Contains 3 IQ domains.
    Contains 1 Ras-GAP domain.
    Contains 1 WW domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • IQ motif containing GTPase activating protein 2 antibody
    • IQGA2_HUMAN antibody
    • Iqgap2 antibody
    • Ras GTPase activating-like protein antibody
    • Ras GTPase-activating-like protein IQGAP2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human colonic carcinoma tissue labeling IQGAP2 with ab181127 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

  • All lanes : Anti-IQGAP2 antibody [EPR13382] (ab181127) at 1/10000 dilution

    Lane 1 : HepG2 lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  • Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling IQGAP2 with ab181127 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

  • Immunohistochemical analysis of paraffin embedded Human liver tissue labeling IQGAP2 ab181127 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

References

ab181127 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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