Overview

  • Product name
  • Description
    Mouse polyclonal to IQGAP3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length Human protein (NP_839943.2)

  • Positive control
    • Placenta

Properties

Applications

Our Abpromise guarantee covers the use of ab88353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 185 kDa.

Target

  • Sequence similarities
    Contains 1 CH (calponin-homology) domain.
    Contains 4 IQ domains.
    Contains 1 Ras-GAP domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • IQ motif containing GTPase activating protein 3 antibody
    • IQGA3_HUMAN antibody
    • IQGAP 3 antibody
    • IQGAP3 antibody
    • MGC10170 antibody
    • MGC10831 antibody
    • MGC1947 antibody
    • OTTHUMP00000031854 antibody
    • OTTHUMP00000031855 antibody
    • Ras GTPase activating like protein IQGAP3 antibody
    • Ras GTPase-activating-like protein IQGAP3 antibody
    see all

Images

  • Anti-IQGAP3 antibody (ab88353) at 1 µg/ml + Human placenta lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 185 kDa
  • All lanes : Anti-IQGAP3 antibody (ab88353) at 1 µg/ml

    Lane 1 : IQGAP3-transfected 293T cell lysate
    Lane 2 : Untransfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 185 kDa
    Observed band size : 179 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-IQGAP3 antibody (ab88353) at 1/500 dilution

    Lane 1 : Mouse lung whole tissue lysate
    Lane 2 : Mouse lung whole tissue lysate
    Lane 3 : Mouse lung adenocarcinoma whole tissue lysate
    Lane 4 : Mouse lung adenocarcinoma whole tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated rabbit anti-mouse IgG monoclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 185 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)


    Exposure time : 15 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References

ab88353 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (lung adenocarcinoma mouse model tissue biopsy)
Specification
lung adenocarcinoma mouse model tissue biopsy
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 25 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (lung cancer cell lines A549, H23)
Specification
lung cancer cell lines A549, H23
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up