Overview

  • Product nameAnti-IQGAP3 antibody
    See all IQGAP3 primary antibodies
  • Description
    Mouse polyclonal to IQGAP3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length Human protein (NP_839943.2)

  • Positive control
    • Placenta

Properties

Applications

Our Abpromise guarantee covers the use of ab88353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 185 kDa.

Target

  • Sequence similaritiesContains 1 CH (calponin-homology) domain.
    Contains 4 IQ domains.
    Contains 1 Ras-GAP domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • IQ motif containing GTPase activating protein 3 antibody
    • IQGA3_HUMAN antibody
    • IQGAP 3 antibody
    • IQGAP3 antibody
    • MGC10170 antibody
    • MGC10831 antibody
    • MGC1947 antibody
    • OTTHUMP00000031854 antibody
    • OTTHUMP00000031855 antibody
    • Ras GTPase activating like protein IQGAP3 antibody
    • Ras GTPase-activating-like protein IQGAP3 antibody
    see all

Anti-IQGAP3 antibody images

  • Anti-IQGAP3 antibody (ab88353) at 1 µg/ml + Human placenta lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 185 kDa
  • All lanes : Anti-IQGAP3 antibody (ab88353) at 1 µg/ml

    Lane 1 : IQGAP3-transfected 293T cell lysate
    Lane 2 : Untransfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 185 kDa
    Observed band size : 179 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-IQGAP3 antibody (ab88353) at 1/500 dilution

    Lane 1 : Mouse lung whole tissue lysate
    Lane 2 : Mouse lung whole tissue lysate
    Lane 3 : Mouse lung adenocarcinoma whole tissue lysate
    Lane 4 : Mouse lung adenocarcinoma whole tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated rabbit anti-mouse IgG monoclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 185 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)


    Exposure time : 15 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-IQGAP3 antibody (ab88353)

ab88353 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (lung adenocarcinoma mouse model tissue biopsy)
Specification lung adenocarcinoma mouse model tissue biopsy
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Jul 25 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (lung cancer cell lines A549, H23)
Specification lung cancer cell lines A549, H23
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Jul 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"