The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
FunctionIn addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.
Tissue specificityExpressed in brain, ovary, heart, lung, liver, kidney and leukocytes. Moderate expression was also detected in lung, skeletal muscle, placenta, small intestine, pancreas, spleen and testis.
Sequence similaritiesBelongs to the BRAG family. Contains 1 IQ domain. Contains 1 PH domain. Contains 1 SEC7 domain.
Cellular localizationCytoplasm. Nucleus. At steady state, may be preferentially cytosolic.
ab122442, at 4 µg/ml, staining IQSEC1 in Human A431 cell line, in green, by Immunoflouroscence. Fixation/Permeabilization: PFA/Triton X-100.
References for Anti-IQSEC1 antibody (ab122442)
This product has been referenced in:
Humphreys D et al. Arf6 coordinates actin assembly through the WAVE complex, a mechanism usurped by Salmonella to invade host cells. Proc Natl Acad Sci U S A110:16880-5 (2013).
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