Overview

  • Product name
  • Description
    Rabbit polyclonal to IQSEC1
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: PSSETGTSLD SPSAYPQGPL VPGSSLSPDH YEHTSVGAYG LYSGPPGQQQ RTRRPKLQHS TSILRKQAE, corresponding to N terminal amino acids 28-96 of Human IQSEC1 protein (UniProt ID: Q6DN90).

  • Positive control
    • Human skin tissue; Human cell line A431

Properties

Applications

Our Abpromise guarantee covers the use of ab122442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Target

  • Function
    In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.
  • Tissue specificity
    Expressed in brain, ovary, heart, lung, liver, kidney and leukocytes. Moderate expression was also detected in lung, skeletal muscle, placenta, small intestine, pancreas, spleen and testis.
  • Sequence similarities
    Belongs to the BRAG family.
    Contains 1 IQ domain.
    Contains 1 PH domain.
    Contains 1 SEC7 domain.
  • Cellular localization
    Cytoplasm. Nucleus. At steady state, may be preferentially cytosolic.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP-ribosylation factors guanine nucleotide-exchange protein 100 antibody
    • ADP-ribosylation factors guanine nucleotide-exchange protein 2 antibody
    • ARF GEP100 antibody
    • ARFGEP100 antibody
    • BRAG2 antibody
    • Brefeldin A resistant ARF GEF2 antibody
    • Brefeldin-resistant Arf-GEF 2 protein antibody
    • GEP100 antibody
    • IQ motif and Sec7 domain 1 antibody
    • IQ motif and SEC7 domain-containing protein 1 antibody
    • IQEC1_HUMAN antibody
    • Iqsec1 antibody
    • Kiaa0763 antibody
    see all

Images

  • ab122442, at a 1/50 dilution, staining IQSEC1 in paraffin embedded Human skin tissue by Immunohistochemistry.
  • ab122442, at 4 µg/ml, staining IQSEC1 in Human A431 cell line, in green, by Immunoflouroscence.
    Fixation/Permeabilization: PFA/Triton X-100.

References

This product has been referenced in:
  • Humphreys D  et al. Arf6 coordinates actin assembly through the WAVE complex, a mechanism usurped by Salmonella to invade host cells. Proc Natl Acad Sci U S A 110:16880-5 (2013). WB ; Human coronavirus . Read more (PubMed: 24085844) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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