Overview

  • Product nameAnti-IRAK antibody [3F7]
    See all IRAK primary antibodies
  • Description
    Mouse monoclonal [3F7] to IRAK
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 530-693 of Human IRAK with a proprietary tag (AAH54000).

  • Positive control
    • Recombinant Human IRAK protein; HeLa cells; K562 cell lysate; Human Placenta and Thyroid tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab119289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 77 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionBinds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
  • Tissue specificityIsoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
    Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'.
  • Information by UniProt
  • Database links
  • Alternative names
    • Il1rak antibody
    • Interleukin 1 receptor associated kinase 1 antibody
    • Interleukin-1 receptor-associated kinase 1 antibody
    • IRAK antibody
    • IRAK-1 antibody
    • Irak1 antibody
    • IRAK1_HUMAN antibody
    • mPLK antibody
    • OTTHUMP00000026014 antibody
    • OTTHUMP00000026015 antibody
    • OTTHUMP00000026020 antibody
    • OTTHUMP00000180621 antibody
    • Pelle antibody
    • Pelle homolog antibody
    • Pelle-like protein kinase antibody
    • Plpk antibody
    see all

Anti-IRAK antibody [3F7] images



  • Predicted band size : 77 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: IRAK1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: Hek293 whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab119289 observed at 85 kDa. Red - loading control, ab181602, observed at 37 kDa. 

    ab119289 was shown to specifically react with IRAK1 when IRAK1 knockout samples were used. Wild-type and IRAK1 knockout samples were subjected to SDS-PAGE. Ab119289 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti-Mouse and 680CW Goat anti-Rabbit secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • ab119289 at 5 µg/ml staining IRAK in Formalin-Fixed, Paraffin-Embedded Human Placenta tissue by Immunohistochemistry.
  • ab119289 at 5 µg/ml staining IRAK in Formalin-Fixed, Paraffin-Embedded Human Thyroid tissue by Immunohistochemistry.
  • Anti-IRAK antibody [3F7] (ab119289) + K562 cell lysate

    Predicted band size : 77 kDa
  • ab119289 at 10 µg/ml staining IRAK in HeLa cells by Immunofluorescence.
  • The detection limit for recombinant tagged IRAK is approximately 0.1 ng/ml when ab119289 is used as a capture antibody.

References for Anti-IRAK antibody [3F7] (ab119289)

ab119289 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8)
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Submitted Aug 22 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"