• Product name
  • Description
    Rabbit polyclonal to IRAK
  • Specificity
    No cross reactivity to IRAK2.
  • Tested applications
    Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Rhesus monkey
  • Immunogen

    Synthetic peptide, corresponding to C terminal amino acids 700-712 of Human IRAK

  • Positive control
    • THP-1 or HeLa whole cell lysate.



Our Abpromise guarantee covers the use of ab238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 80 kDa.Can be blocked with IRAK peptide (ab6230). (THP-1 or HeLa whole cell) from non-activated cells.
IP Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.


  • Function
    Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
  • Tissue specificity
    Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
    Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA48924 antibody
    • Il1rak antibody
    • Interleukin 1 receptor associated kinase 1 antibody
    • Interleukin-1 receptor-associated kinase 1 antibody
    • IRAK antibody
    • IRAK-1 antibody
    • Irak1 antibody
    • IRAK1-S antibody
    • IRAK1_HUMAN antibody
    • mPLK antibody
    • OTTHUMP00000026014 antibody
    • OTTHUMP00000026015 antibody
    • OTTHUMP00000026020 antibody
    • OTTHUMP00000180621 antibody
    • Pelle antibody
    • Pelle homolog antibody
    • Pelle-like protein kinase antibody
    • Plpk antibody
    see all


  • All lanes : Anti-IRAK antibody (ab238) at 1/2000 dilution

    Lane 1 : THP-1 (THP)whole cell lysate
    Lane 2 : HeLa (HL) whole cell lysate

    Predicted band size : 80 kDa
    Observed band size : 80 kDa
  • ab238 at 10µg/ml staining Hela cells by ICC/IF
  • IHC image of ab238 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab238, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab238 staining IRAK in Human platelet cells by Flow cytometry.
    Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/200 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.

    P : Permeabilized
    US : Unstained (Red Peak)
    IGG RB : IgG Rabbit (Blue Peak)
    IRAK Ab (Green Peak)

    See Abreview

  • ab238 staining IRAK in murine RAW 264.7 cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 22°C and then incubated with ab238 at a 1/150 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.

    See Abreview

  • IRAK was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to IRAK and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab238.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 80kDa: IRAK
  • Immunofluorescence of IRAK in HeLa cells using ab238 at 20 ug/ml.


This product has been referenced in:
  • Berger EA  et al. ß-Adrenergic receptor agonist, compound 49b, inhibits TLR4 signaling pathway in diabetic retina. Immunol Cell Biol 94:656-61 (2016). WB ; Rat . Read more (PubMed: 26888251) »
  • Kishi N  et al. Reduction of aberrant NF-?B signalling ameliorates Rett syndrome phenotypes in Mecp2-null mice. Nat Commun 7:10520 (2016). ICC . Read more (PubMed: 26821816) »

See all 4 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Rhesus monkey Cell (Colon)
Yes - 0.1% Triton-x-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 21 2013

I have checked your new order 1217270 and it looks that both antibodies (abhttp://ops.adminsite.com/admin/data/fs_ab.cfm?&intAbID=119289 and abhttp://ops.adminsite.com/admin/data/fs_ab.cfm?&intAbID=135027) are out of stock at the moment. We hav...

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Thank you for getting back to me.

This is to confirm that I have placed a new order for you - for one vial of ab238 and another vial of ab64171 as free of charge replacements and the new order number is 1217226.

I would kindly advis...

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Thank you for getting back to me and for passing some useful details. Your co-operation is much appreciated.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data shee...

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Thank you for getting back to me.

I can find the 3 pdf files attached to your response but it seems that the ppt (Powerpoint) file did not come through.

After reading through the detailed protocol you kindly forwarded to Abcam, I wo...

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I am sorry to hear that you have been experiencing problems using these two products (ab238 and ab64171) in the application that you wish. Thank you very much for sending the WB images.

In order to assess the quality of our products I would a...

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Thank you for your inquiry.

I can confirm that the protocol for flow cytometry that we know of for this antibody includes an incubation time of 16 hours at 4C.

Please feel free to use your standard protocol if you find that this is ...

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I am sorry to hear that the result did not improve.

To proceed further in this case could you let me know, how the antibody was stored and what was the purchase order number?

Thank very much for your cooperation in this case!

Thank you for submitting an Abreview of ab238. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on th...

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Western blot
Mouse Cell lysate - whole cell (RAW macrophages cell line)
Loading amount
50 µg
RAW macrophages cell line
LPS and peptidoglycan
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Varkha Agrawal

Verified customer

Submitted Sep 11 2012

1-10 of 21 Abreviews or Q&A


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