Overview

  • Product nameAnti-IRAK1BP1 antibody
    See all IRAK1BP1 primary antibodies
  • Description
    Mouse polyclonal to IRAK1BP1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length IRAK1BP1 protein (Human)

  • Positive control
    • IRAK1BP1 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa).

Target

  • RelevanceIRAK1BP1 is a component of the IRAK1 dependent TNFRSF1A signaling pathway that leads to NF-kappa-B activation and is required for cell survival. It acts by enhancing RELA transcriptional activity.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • ActA binding protein 3 antibody
    • AIP70 antibody
    • Interleukin 1 receptor associated kinase 1 binding protein 1 antibody
    • IRAK1-binding protein 1 antibody
    • Signaling molecule that associates with Pelle-like kinase antibody
    • SIMPL antibody
    see all

Anti-IRAK1BP1 antibody images

  • All lanes : Anti-IRAK1BP1 antibody (ab67841) at 1/500 dilution

    Lane 1 : IRAK1BP1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 29 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

References for Anti-IRAK1BP1 antibody (ab67841)

ab67841 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (macrophages (Raw 264.7))
Loading amount 100 µg
Specification macrophages (Raw 264.7)
Treatment untreated + LPS-treated (10 ng/ml)
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jul 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"