Overview

  • Product name
  • Description
    Rabbit polyclonal to IRAK2
  • Host species
    Rabbit
  • Specificity
    ab25957 recognises IRAK2 but does not cross-react with IRAK.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide, corresponding to 16 C terminal amino acids of Mouse IRAK2a isoform. This sequence is common to all four isoforms.

  • Positive control
    • Whole cell lysate from A-20 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab25957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 65 kDa.

Target

  • Function
    Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
  • Tissue specificity
    Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Domain
    The protein kinase domain is predicted to be catalytically inactive.
  • Information by UniProt
  • Database links
  • Alternative names
    • il1rak2 antibody
    • Interleukin 1 receptor associated kinase 2 antibody
    • Interleukin 1 receptor associated kinase like 2 antibody
    • Interleukin-1 receptor-associated kinase-like 2 antibody
    • IRAK 2 antibody
    • IRAK-2 antibody
    • Irak2 antibody
    • IRAK2_HUMAN antibody
    • MGC150550 antibody
    see all

Images

  • Lane 1 : Anti-IRAK2 antibody (ab25957) at 0.5 µg/ml
    Lane 2 : Anti-IRAK2 antibody (ab25957) at 1 µg/ml
    Lane 3 : Anti-IRAK2 antibody (ab25957) at 2 µg/ml

    All lanes : A-20 whole cell lysate

    Predicted band size: 65 kDa

References

ab25957 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (LIVER)
Specification
LIVER
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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Verified customer

Submitted Nov 10 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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