Anti-IRAK2 antibody (ab6148)

Overview

  • Product nameAnti-IRAK2 antibody
    See all IRAK2 primary antibodies
  • Description
    Rabbit polyclonal to IRAK2
  • SpecificityAnti-IRAK2 has no cross response to IRAK.
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 546-564 of Human IRAK2.

  • Positive control
    • Whole cell lysate from HeLa or K562 cells.
  • General notes


    The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPBS with 0.02% sodium azide
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesPurified IgG was prepared by affinity chromatography.
  • Primary antibody notesThe pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab6148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 2 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 65 kDa.Can be blocked with IRAK2 peptide (ab6232).
IP Use at 1 µg/mg of lysate.

Target

  • FunctionBinds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
  • Tissue specificityExpressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • DomainThe protein kinase domain is predicted to be catalytically inactive.
  • Information by UniProt
  • Database links
  • Alternative names
    • il1rak2 antibody
    • Interleukin 1 receptor associated kinase 2 antibody
    • Interleukin 1 receptor associated kinase like 2 antibody
    • Interleukin-1 receptor-associated kinase-like 2 antibody
    • IRAK 2 antibody
    • IRAK-2 antibody
    • Irak2 antibody
    • IRAK2_HUMAN antibody
    • MGC150550 antibody
    see all

Anti-IRAK2 antibody images

  • All lanes : Anti-IRAK2 antibody (ab6148) at 2 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : K562 whole cell lysate


    Predicted band size : 65 kDa
    Observed band size : 65 kDa
  • ab6148 at 2µg/ml staining IRAK2 in Hela cells by ICC/IF

  • Immunofluorescence of IRAK-2 in Hela cells using ab6148 at 10 ug/ml.

References for Anti-IRAK2 antibody (ab6148)

ab6148 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Total protein in input 1000 µg
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Verified customer

Submitted Nov 07 2013

We do not have any reports of this antibody being used for immunoprecipitation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"