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Synthetic peptide, corresponding to amino acids 571-590 of Human IRAK2.
Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. The long-sought IkB kinase (IKK),which phosphorylates IkB, and mediates IkB degradation and NF-kB activation, was recently identified by several laboratories (1-5). IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKa and IKKb). IKKa and IKKb interact with each other and both are essential for NF-kB activation. IKKb phosphorylates both IkB-a and IkB-b. IKKb is expressed in a variety of human tissues.
Our Abpromise guarantee covers the use of ab6158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 65 kDa.Can be blocked with IRAK2 peptide (ab6233).|
ab6158 at 10µg/ml staining IRAK-2 in Hela cells by ICC/IF
ab6158 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"