Overview

  • Product nameAnti-IRAK2 antibody
    See all IRAK2 primary antibodies
  • Description
    Rabbit polyclonal to IRAK2
  • SpecificityThis antibody reacts with IRAK2, it does not react with IRAK
  • Tested applicationsSuitable for: ICC/IF, ICC, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to 17 amino acids near the C terminus of mouse IRAK2a, (which is common to all four isoforms).

  • Positive control
    • RAW2647 whole cell lysate and A20 cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
ICC
IP
WB
  • Application notesICC: Use at a concentration of 1 µg/ml.
    IP: Use at an assay dependent dilution (PMID 19224918).
    WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
    • Tissue specificityExpressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
    • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
      Contains 1 death domain.
      Contains 1 protein kinase domain.
    • DomainThe protein kinase domain is predicted to be catalytically inactive.
    • Information by UniProt
    • Database links
    • Alternative names
      • il1rak2 antibody
      • Interleukin 1 receptor associated kinase 2 antibody
      • Interleukin 1 receptor associated kinase like 2 antibody
      • Interleukin-1 receptor-associated kinase-like 2 antibody
      • IRAK 2 antibody
      • IRAK-2 antibody
      • Irak2 antibody
      • IRAK2_HUMAN antibody
      • MGC150550 antibody
      see all

    Anti-IRAK2 antibody images


    • Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 65 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)


      Exposure time : 15 seconds

      This image is courtesy of an anonymous abreview.

      Anti-IRAK antibody (ab62419) reactivity with reduced wildtype (WT) or IRAK2 knockout (KO) mouse embryonic fibroblast (MEF) cell lysate. After SDS-PAGE, membranes were blocked in 5% milk for 1h at 25°C before incubation with ab62419 (1µg/ml dilution in 5% milk in TBS/0.5% Tween) for 16h at 4ºC. The blot was then incubated with an anti-Rabbit HRP-conjugated secondary antibody before developing with ECL.

      See Abreview

    • Lane 1 : Anti-IRAK2 antibody (ab62419) at 0.5 µg/ml
      Lane 2 : Anti-IRAK2 antibody (ab62419) at 1 µg/ml
      Lane 3 : Anti-IRAK2 antibody (ab62419) at 2 µg/ml

      Lane 1 : RAW264.7 whole cell lysate
      Lane 2 : RAW264.7 whole cell lysate
      Lane 3 : RAW264.7 whole cell lysate

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 65 kDa
      Observed band size : 65 kDa
    • Immunocytochemistry of IRAK2 in A-20 cells with ab62419 antibody at 1 µg/ml.
    • Immunofluorescence of IRAK2 in A20 cells using ab62419 at 10 ug/ml.

    References for Anti-IRAK2 antibody (ab62419)

    This product has been referenced in:
    • Martin BN  et al. IKKa negatively regulates ASC-dependent inflammasome activation. Nat Commun 5:4977 (2014). Read more (PubMed: 25266676) »
    • Xu H  et al. Notch-RBP-J signaling regulates the transcription factor IRF8 to promote inflammatory macrophage polarization. Nat Immunol 13:642-50 (2012). WB, IP . Read more (PubMed: 22610140) »

    See all 5 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (8%)
    Sample Mouse Cell lysate - whole cell (MEFs)
    Specification MEFs
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 09 2013

    Application Immunoprecipitation
    Immuno-precipitation step Protein G
    Sample Human Cell lysate - whole cell (HEK293)
    Specification HEK293
    Total protein in input 500 µg
    Username

    Abcam user community

    Verified customer

    Submitted Sep 09 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"