Overview

  • Product name
  • Description
    Rabbit polyclonal to IRAK2
  • Specificity
    This antibody reacts with IRAK2, it does not react with IRAK
  • Tested applications
    Suitable for: ICC/IF, ICC, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to 17 amino acids near the C terminus of mouse IRAK2a, (which is common to all four isoforms).

  • Positive control
    • RAW2647 whole cell lysate and A20 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab62419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
ICC
IP
WB
  • Application notes
    ICC: Use at a concentration of 1 µg/ml.
    IP: Use at an assay dependent dilution (PMID 19224918).
    WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
    • Tissue specificity
      Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
    • Sequence similarities
      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
      Contains 1 death domain.
      Contains 1 protein kinase domain.
    • Domain
      The protein kinase domain is predicted to be catalytically inactive.
    • Information by UniProt
    • Database links
    • Alternative names
      • il1rak2 antibody
      • Interleukin 1 receptor associated kinase 2 antibody
      • Interleukin 1 receptor associated kinase like 2 antibody
      • Interleukin-1 receptor-associated kinase-like 2 antibody
      • IRAK 2 antibody
      • IRAK-2 antibody
      • Irak2 antibody
      • IRAK2_HUMAN antibody
      • MGC150550 antibody
      see all

    Images


    • Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 65 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)


      Exposure time : 15 seconds

      This image is courtesy of an anonymous abreview.

      Anti-IRAK antibody (ab62419) reactivity with reduced wildtype (WT) or IRAK2 knockout (KO) mouse embryonic fibroblast (MEF) cell lysate. After SDS-PAGE, membranes were blocked in 5% milk for 1h at 25°C before incubation with ab62419 (1µg/ml dilution in 5% milk in TBS/0.5% Tween) for 16h at 4ºC. The blot was then incubated with an anti-Rabbit HRP-conjugated secondary antibody before developing with ECL.

      See Abreview

    • Lane 1 : Anti-IRAK2 antibody (ab62419) at 0.5 µg/ml
      Lane 2 : Anti-IRAK2 antibody (ab62419) at 1 µg/ml
      Lane 3 : Anti-IRAK2 antibody (ab62419) at 2 µg/ml

      Lane 1 : RAW264.7 whole cell lysate
      Lane 2 : RAW264.7 whole cell lysate
      Lane 3 : RAW264.7 whole cell lysate

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 65 kDa
      Observed band size : 65 kDa
    • Immunocytochemistry of IRAK2 in A-20 cells with ab62419 antibody at 1 µg/ml.
    • Immunofluorescence of IRAK2 in A20 cells using ab62419 at 10 ug/ml.

    References

    This product has been referenced in:
    • McGuire VA  et al. Dimethyl fumarate blocks pro-inflammatory cytokine production via inhibition of TLR induced M1 and K63 ubiquitin chain formation. Sci Rep 6:31159 (2016). Read more (PubMed: 27498693) »
    • Sun J  et al. Comprehensive RNAi-based screening of human and mouse TLR pathways identifies species-specific preferences in signaling protein use. Sci Signal 9:ra3 (2016). Read more (PubMed: 26732763) »

    See all 7 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Mouse Cell lysate - whole cell (MEFs)
    Specification
    MEFs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 09 2013

    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein G
    Sample
    Human Cell lysate - whole cell (HEK293)
    Specification
    HEK293
    Total protein in input
    500 µg
    Username

    Abcam user community

    Verified customer

    Submitted Sep 09 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up