Overview

  • Product name
  • Description
    Mouse monoclonal to IRAK2
  • Tested applications
    Suitable for: ELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Recombinant fragment with tag: KPEKPLAASV RKAEDEQEEG QPVRMATFPG PGSSPARAHQ PAFLQPPEED APHSLRSDLP TSSDSKDFST SIPKQEKLLS LAGDSLFWSE ADVVQATDDF , corresponding to amino acids 111-210 of Human IRAK2

  • Positive control
    • HeLa cell lysate.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab66017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
IP
  • Application notes
    ELISA: Use at an assay dependent dilution. Sandwich ELISA: The detection limit for recombinant tagged IRAK2 is approximately 0.03ng/ml as a capture antibody.
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
    • Tissue specificity
      Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
    • Sequence similarities
      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
      Contains 1 death domain.
      Contains 1 protein kinase domain.
    • Domain
      The protein kinase domain is predicted to be catalytically inactive.
    • Information by UniProt
    • Database links
    • Alternative names
      • il1rak2 antibody
      • Interleukin 1 receptor associated kinase 2 antibody
      • Interleukin 1 receptor associated kinase like 2 antibody
      • Interleukin-1 receptor-associated kinase-like 2 antibody
      • IRAK 2 antibody
      • IRAK-2 antibody
      • Irak2 antibody
      • IRAK2_HUMAN antibody
      • MGC150550 antibody
      see all

    Anti-IRAK2 antibody images

    • Anti-IRAK2 antibody (ab66017) at 1 µg/ml + HeLa cell lysate at 50 µg/ml

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 65 kDa
      Observed band size : 65 kDa

    References for Anti-IRAK2 antibody (ab66017)

    ab66017 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein G
    Sample
    Human Cell lysate - whole cell (HEK293)
    Specification
    HEK293
    Total protein in input
    1000 µg
    Username

    Abcam user community

    Verified customer

    Submitted Nov 07 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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