Overview

  • Product nameAnti-IRAK2 antibody
    See all IRAK2 primary antibodies
  • Description
    Mouse monoclonal to IRAK2
  • Tested applicationsSuitable for: ELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Recombinant fragment with tag: KPEKPLAASV RKAEDEQEEG QPVRMATFPG PGSSPARAHQ PAFLQPPEED APHSLRSDLP TSSDSKDFST SIPKQEKLLS LAGDSLFWSE ADVVQATDDF , corresponding to amino acids 111-210 of Human IRAK2

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab66017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
IP
  • Application notesELISA: Use at an assay dependent dilution. Sandwich ELISA: The detection limit for recombinant tagged IRAK2 is approximately 0.03ng/ml as a capture antibody.
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
    • Tissue specificityExpressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
    • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
      Contains 1 death domain.
      Contains 1 protein kinase domain.
    • DomainThe protein kinase domain is predicted to be catalytically inactive.
    • Information by UniProt
    • Database links
    • Alternative names
      • il1rak2 antibody
      • Interleukin 1 receptor associated kinase 2 antibody
      • Interleukin 1 receptor associated kinase like 2 antibody
      • Interleukin-1 receptor-associated kinase-like 2 antibody
      • IRAK 2 antibody
      • IRAK-2 antibody
      • Irak2 antibody
      • IRAK2_HUMAN antibody
      • MGC150550 antibody
      see all

    Anti-IRAK2 antibody images

    • Anti-IRAK2 antibody (ab66017) at 1 µg/ml + HeLa cell lysate at 50 µg/ml

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 65 kDa
      Observed band size : 65 kDa

    References for Anti-IRAK2 antibody (ab66017)

    ab66017 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunoprecipitation
    Immuno-precipitation step Protein G
    Sample Human Cell lysate - whole cell (HEK293)
    Specification HEK293
    Total protein in input 1000 µg
    Username

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    Verified customer

    Submitted Nov 07 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"