Anti-IRAK2 antibody - Carboxyterminal end (ab47995)

Overview

  • Product nameAnti-IRAK2 antibody - Carboxyterminal end
    See all IRAK2 primary antibodies
  • Description
    Rabbit polyclonal to IRAK2 - Carboxyterminal end
  • Specificityab47995 is directed against human IRAK 2 protein. No cross reactivity occurs against IRAK 1.
  • Tested applicationsSuitable for: ELISA, WB, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to a region near the carboxy terminus of human IRAK-2 protein.

  • Positive control
    • HeLa and K562 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab47995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
ICC
  • Application notesELISA: 1/5000 - 1/20000.
    ICC (PFA fixed): 1/300 - 1/600.
    WB: 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
    • Tissue specificityExpressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
    • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
      Contains 1 death domain.
      Contains 1 protein kinase domain.
    • DomainThe protein kinase domain is predicted to be catalytically inactive.
    • Information by UniProt
    • Database links
    • Alternative names
      • il1rak2 antibody
      • Interleukin 1 receptor associated kinase 2 antibody
      • Interleukin 1 receptor associated kinase like 2 antibody
      • Interleukin-1 receptor-associated kinase-like 2 antibody
      • IRAK 2 antibody
      • IRAK-2 antibody
      • Irak2 antibody
      • IRAK2_HUMAN antibody
      • MGC150550 antibody
      see all

    Anti-IRAK2 antibody - Carboxyterminal end images

    • All lanes : Anti-IRAK2 antibody - Carboxyterminal end (ab47995) at 1/500 dilution

      Lane 1 : (H): HeLa whole cell lysates.
      Lane 2 : (K): K562 whole cell lysates.

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 65 kDa
      Observed band size : 65 kDa
      Additional bands at : 40 kDa,43 kDa,56 kDa. We are unsure as to the identity of these extra bands.
    • Staining of IRAK-2 in HeLa cells. The cells were fixed with paraformaldehyde and reacted with the primary antibody ab47995 diluted at 1:500.

    References for Anti-IRAK2 antibody - Carboxyterminal end (ab47995)

    ab47995 has not yet been referenced specifically in any publications.

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