Recombinant
RabMAb

Anti-IRAK2 antibody [EPR16530] (ab192249)

Overview

  • Product name
    Anti-IRAK2 antibody [EPR16530]
    See all IRAK2 primary antibodies
  • Description
    Rabbit monoclonal [EPR16530] to IRAK2
  • Tested applications
    Suitable for: Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human IRAK2 aa 50-200. The exact sequence is proprietary.
    Database link: O43187

  • Positive control
    • K562, MOLT4, THP1 and HeLa cell lysates; MOLT4 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab192249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/220. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/60.
WB 1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).

Target

  • Function
    Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
  • Tissue specificity
    Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Domain
    The protein kinase domain is predicted to be catalytically inactive.
  • Information by UniProt
  • Database links
  • Alternative names
    • il1rak2 antibody
    • Interleukin 1 receptor associated kinase 2 antibody
    • Interleukin 1 receptor associated kinase like 2 antibody
    • Interleukin-1 receptor-associated kinase-like 2 antibody
    • IRAK 2 antibody
    • IRAK-2 antibody
    • Irak2 antibody
    • IRAK2_HUMAN antibody
    • MGC150550 antibody
    see all

Images

  • All lanes : Anti-IRAK2 antibody [EPR16530] (ab192249) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : MOLT4 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 69 kDa

    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed MOLT4 cells labeling IRAK2 with ab192249 at 1/220 dilution (pink), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • All lanes : Anti-IRAK2 antibody [EPR16530] (ab192249) at 1/1000 dilution

    Lane 1 : THP1 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 69 kDa

    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Western blot analysis of IRAK2 in MOLT4 cell lysates immunoprecipitated using ab192249 at 1/60 dilution. 

     

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

    Blocking/Dilution buffer: 5% NFDM /TBST.

References

ab192249 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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