Overview

  • Product name
  • Description
    Goat polyclonal to IRAK4
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Horse, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    NKPITPSTYVRC

    conjugated to KLH, corresponding to N terminal amino acids 2-13 of Human IRAK4.

  • Positive control
    • Human heart tissue; Human liver tissue; HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab113525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 52 kDa.
ELISA 1/32000.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
  • Involvement in disease
    Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
    Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • IL-1 receptor-associated kinase 4 antibody
    • Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
    • Interleukin-1 receptor-associated kinase 4 antibody
    • Interleukin1 receptor associated kinase 4 antibody
    • IPD1 antibody
    • IRAK 4 antibody
    • IRAK-4 antibody
    • IRAK4 antibody
    • IRAK4 mutated form 1 antibody
    • IRAK4_HUMAN antibody
    • LOC 51135 antibody
    • NY REN 64 antibody
    • NY REN 64 antigen antibody
    • NY-REN-64 antibody
    • REN64 antibody
    • Renal carcinoma antigen NY-REN-64 antibody
    see all

Images

  • Anti-IRAK4 antibody (ab113525) at 1/1 dilution + HeLa cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 52 kDa

  • ab113525 at 3.75ug/ml staining IRAK4 in Human heart tissue by immunohistochemistry.
  • ab113525 at 3.75ug/ml staining IRAK4 in Human liver tissue by immunohistochemistry.

References

ab113525 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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