Overview

  • Product nameAnti-IRAK4 antibody
    See all IRAK4 primary antibodies
  • Description
    Mouse monoclonal to IRAK4
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-130 of Human IRAK4, with 26 kDa tag. AAH13316.

  • Positive control
    • Recombinant protein, K562 cell lysate and human testis tissue sections.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 35, 51 kDa (predicted molecular weight: 51 kDa).
ELISA Use at an assay dependent dilution.
IHC-P Use a concentration of 6 µg/ml.

Target

  • FunctionRequired for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
  • Involvement in diseaseDefects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
    Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • IL-1 receptor-associated kinase 4 antibody
    • Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
    • Interleukin-1 receptor-associated kinase 4 antibody
    • Interleukin1 receptor associated kinase 4 antibody
    • IPD1 antibody
    • IRAK 4 antibody
    • IRAK-4 antibody
    • IRAK4 antibody
    • IRAK4 mutated form 1 antibody
    • IRAK4_HUMAN antibody
    • LOC 51135 antibody
    • NY REN 64 antibody
    • NY REN 64 antigen antibody
    • NY-REN-64 antibody
    • REN64 antibody
    • Renal carcinoma antigen NY-REN-64 antibody
    see all

Anti-IRAK4 antibody images

  • Anti-IRAK4 antibody (ab88124) at 5 µg/ml + recombinant protein (immunogen) at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 51 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Anti-IRAK4 antibody (ab88124) at 1/500 dilution + K562 cell lysate at 50 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
  • ab88124 at 6µg/ml staining IRAK4 in human testis tissue.
  • Detection limit for ab88124 is approximately 1ng/ml as a capture antibody.

References for Anti-IRAK4 antibody (ab88124)

ab88124 has not yet been referenced specifically in any publications.

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