Anti-IRAK4 (phospho T345) antibody [A8A8] (ab113656)

Overview

  • Product nameAnti-IRAK4 (phospho T345) antibody [A8A8]
    See all IRAK4 primary antibodies
  • Description
    Mouse monoclonal [A8A8] to IRAK4 (phospho T345)
  • Specificityab113656 does not cross-react with non-phosphospecific peptide.
  • Tested applicationsSuitable for: WB, IP, IHC-P, ELISA, Dot blot, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog
  • Immunogen

    Synthetic peptide surrounding to the epitope -

    VMTSR

    - of Human IRAK4.

  • Positive control
    • Jurkat Cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab113656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 52 kDa).
IP Use a concentration of 2 - 5 µg/ml.
IHC-P Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.

Tested with immunizing peptide in peptide ELISA.

Dot blot 1/1000.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionRequired for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
  • Involvement in diseaseDefects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
    Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • IL-1 receptor-associated kinase 4 antibody
    • Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
    • Interleukin-1 receptor-associated kinase 4 antibody
    • Interleukin1 receptor associated kinase 4 antibody
    • IPD1 antibody
    • IRAK 4 antibody
    • IRAK-4 antibody
    • IRAK4 antibody
    • IRAK4 mutated form 1 antibody
    • IRAK4_HUMAN antibody
    • LOC 51135 antibody
    • NY REN 64 antibody
    • NY REN 64 antigen antibody
    • NY-REN-64 antibody
    • REN64 antibody
    • Renal carcinoma antigen NY-REN-64 antibody
    see all

Anti-IRAK4 (phospho T345) antibody [A8A8] images

  • All lanes : Anti-IRAK4 (phospho T345) antibody [A8A8] (ab113656) at 1/500 dilution

    Lane 1 : Jurkat cells stimulated by 50 ng/ml IL1 (10 min)
    Lane 2 : Jurkat cells stimulated by 50 ng/ml IL1 (10 min) with phospho specific peptide
    Lane 3 : Jurkat cells stimulated by 50 ng/ml IL1 (10 min) with non-phospho specific peptide


    Predicted band size : 52 kDa
  • Dot clot using ab113656 at 1/1000 dilution:
    A: 1µg IRAK4(pT345) peptide
    B: 1µg IRAK4(non phosphorylated) peptide
  • Overlay histogram showing K562 cells stained with ab113656 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab113656, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-IRAK4 (phospho T345) antibody [A8A8] (ab113656)

ab113656 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab113656.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"