Overview

  • Product nameAnti-IRAKM antibody
    See all IRAKM primary antibodies
  • Description
    Mouse monoclonal to IRAKM
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment: RIDRMTQKTP FECSQSEVMF LSLDKKPESK RNEEACNMPS SSCEESWFPK , corresponding to amino acids 497-596 of Human IRAKM (AAH57800) with tag. (MW of the tag alone is 26 KDa).

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged IRAK3 is approximately 0.3ng/ml when used as a capture antibody.

Target

  • FunctionInhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.
  • Tissue specificityExpressed predominantly in peripheral blood lymphocytes.
  • Involvement in diseaseDefects in IRAK3 are associated with susceptibility to asthma-related traits type 5 (ASRT5) [MIM:611064]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASRT5 antibody
    • IL 1 receptor associated kinase M antibody
    • IL-1 receptor-associated kinase M antibody
    • IL1 receptor associated kinase M antibody
    • Interleukin 1 receptor associated kinase 3 antibody
    • Interleukin 1 receptor associated kinase M antibody
    • Interleukin-1 receptor-associated kinase 3 antibody
    • IRAK M antibody
    • IRAK-3 antibody
    • IRAK-M antibody
    • Irak3 antibody
    • IRAK3_HUMAN antibody
    see all

Anti-IRAKM antibody images

  • Anti-IRAKM antibody (ab77216) at 1/500 dilution + recombinant protein immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 67 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-IRAKM antibody (ab77216) at 1/500 dilution + HeLa cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 67 kDa
    Observed band size : 67 kDa
  • Detection limit for recombinant tagged IRAK3 is approximately 0.3ng/ml as a capture antibody.

References for Anti-IRAKM antibody (ab77216)

ab77216 has not yet been referenced specifically in any publications.

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