Overview

  • Product nameAnti-IRAKM antibody
    See all IRAKM primary antibodies
  • Description
    Rabbit polyclonal to IRAKM
  • SpecificityAnti-IRAK-M has no cross response to IRAK or IRAK2.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human
  • Immunogen

    Synthetic peptide:

    CSSKFSWDEYEQYKKE

    , corresponding to C terminal amino acids 581-596 of Human IRAKM (Peptide available as ab6231.)

  • Positive control
    • Mouse spleen or rat kidney tissue lysate.
  • General notes


    Interleukin-1 (IL-1) and lipopolysaccharide (LPS)induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (Wesche H et al.1999,Muzio M et al.1997), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in theIRAK/Pelle family was recently identified and designated IRAK-M (Cao Z et al.1996). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and thedominant negative mutants of IRAKs inhibit IL-1 orLPS induced NF-kB activation. Members inIRAK/Pelle family play a central role in IL-1R/TLRmediated inflammatory responses to cytokine IL-1and LPS.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Storage bufferPBS with 0.02% sodium azide
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesPurified IgG prepared by ion exchange chromatography. IRAK-M Antibody is affinity chromatography purified via peptide column.
  • Primary antibody notesInterleukin-1 (IL-1) and lipopolysaccharide (LPS)induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (Wesche H et al.1999,Muzio M et al.1997), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in theIRAK/Pelle family was recently identified and designated IRAK-M (Cao Z et al.1996). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and thedominant negative mutants of IRAKs inhibit IL-1 orLPS induced NF-kB activation. Members inIRAK/Pelle family play a central role in IL-1R/TLRmediated inflammatory responses to cytokine IL-1and LPS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab8116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20585389
IHC-P Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Can be blocked with IRAKM peptide (ab6231).

Target

  • FunctionInhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.
  • Tissue specificityExpressed predominantly in peripheral blood lymphocytes.
  • Involvement in diseaseDefects in IRAK3 are associated with susceptibility to asthma-related traits type 5 (ASRT5) [MIM:611064]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASRT5 antibody
    • IL 1 receptor associated kinase M antibody
    • IL-1 receptor-associated kinase M antibody
    • IL1 receptor associated kinase M antibody
    • Interleukin 1 receptor associated kinase 3 antibody
    • Interleukin 1 receptor associated kinase M antibody
    • Interleukin-1 receptor-associated kinase 3 antibody
    • IRAK M antibody
    • IRAK-3 antibody
    • IRAK-M antibody
    • Irak3 antibody
    • IRAK3_HUMAN antibody
    see all

Anti-IRAKM antibody images

  • All lanes : Anti-IRAKM antibody (ab8116) at 1 µg/ml

    Lane 1 : Mouse spleen tissue lysates
    Lane 2 : Rat liver tissue lysate


    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Western blot analysis of IRAKM in mouse spleen (M) and rat liver (R) tissue lysates with anti-IRAKM at 1 µg /ml.
  • Immunohistochemical staining of rat liver tissue using IRAK-M antibody at 2 µg/ml.

  • ab8116 at 2µg/ml staining IRAKM in rat liver cells by IHC  

  • ab8116 staining IRAKM in murine lung macrophages by Immunocytochemistry/ Immunofluorescence.<.br>
    The cells were permeablized with a 1:1 mixture of methanol and acetone. After washing with PBS, ab8116 was applied and the cells incubated for one hour. After washing, antibodies against the primary antibody labeled with FITC were applied to the cells and incubated for one hour. The cell were then mounted in DAPI mounting media and viewed on the fluorescent microscope.
  • Immunofluorescence of IRAK-M in Rat Liver cells using ab8116 at 10 ug/ml.

References for Anti-IRAKM antibody (ab8116)

This product has been referenced in:
  • Udgata A  et al. Transduction of Functionally Contrasting Signals by Two Mycobacterial PPE Proteins Downstream of TLR2 Receptors. J Immunol 197:1776-87 (2016). Read more (PubMed: 27481848) »
  • Hayashi T  et al. Induction of Tolerogenic Dendritic Cells by a PEGylated TLR7 Ligand for Treatment of Type 1 Diabetes. PLoS One 10:e0129867 (2015). Read more (PubMed: 26076454) »

See all 9 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Nov 10 2014

Application Western blot
Loading amount 1e+006 cells
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (primary human macrophage)
Specification primary human macrophage
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Submitted Dec 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (monocyte-derived macrophage)
Loading amount 30 µg
Specification monocyte-derived macrophage
Treatment 100 µg/ml muramyl dipeptide for 48 hrs
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jan 17 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"