Overview

  • Product nameAnti-IRAKM antibody [Y278]
    See all IRAKM primary antibodies
  • Description
    Rabbit monoclonal [Y278] to IRAKM
  • Tested applicationsSuitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in the C-term of human IRAK-3

  • Positive control
    • A431 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50.
WB 1/20000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
  • Application notesIs unsuitable for Flow Cyt,ICC or IHC.
  • Target

    • FunctionInhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.
    • Tissue specificityExpressed predominantly in peripheral blood lymphocytes.
    • Involvement in diseaseDefects in IRAK3 are associated with susceptibility to asthma-related traits type 5 (ASRT5) [MIM:611064]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.
    • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
      Contains 1 death domain.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • ASRT5 antibody
      • IL 1 receptor associated kinase M antibody
      • IL-1 receptor-associated kinase M antibody
      • IL1 receptor associated kinase M antibody
      • Interleukin 1 receptor associated kinase 3 antibody
      • Interleukin 1 receptor associated kinase M antibody
      • Interleukin-1 receptor-associated kinase 3 antibody
      • IRAK M antibody
      • IRAK-3 antibody
      • IRAK-M antibody
      • Irak3 antibody
      • IRAK3_HUMAN antibody
      see all

    Anti-IRAKM antibody [Y278] images



    • Predicted band size : 68 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: IRAKM knockout HAP1 cell lysate (20 µg)

      Lanes 1 - 2: Merged signal (red and green). Green - ab32394 observed at 69 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32394 was shown to specifically react with IRAKM when IRAKM knockout samples were used. Wild-type and IRAKM knockout samples were subjected to SDS-PAGE. ab32394 and ab8245 (loading control to GAPDH) diluted to 1/5000 and 1/10000 respectively were incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-IRAKM antibody [Y278] (ab32394) at 1/20000 dilution + A431 cell lysate

      Predicted band size : 68 kDa
      Observed band size : 68 kDa

    References for Anti-IRAKM antibody [Y278] (ab32394)

    ab32394 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab32394.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"