Validated using a knockout cell line
Recombinant
RabMAb

Anti-IRAKM antibody [Y278] (ab32394)

Overview

  • Product name
    Anti-IRAKM antibody [Y278]
    See all IRAKM primary antibodies
  • Description
    Rabbit monoclonal [Y278] to IRAKM
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in the C-term of human IRAK-3

  • Positive control
    • A431 cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50.
WB 1/20000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC.
  • Target

    • Function
      Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.
    • Tissue specificity
      Expressed predominantly in peripheral blood lymphocytes.
    • Involvement in disease
      Defects in IRAK3 are associated with susceptibility to asthma-related traits type 5 (ASRT5) [MIM:611064]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.
    • Sequence similarities
      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
      Contains 1 death domain.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • ASRT5 antibody
      • IL 1 receptor associated kinase M antibody
      • IL-1 receptor-associated kinase M antibody
      • IL1 receptor associated kinase M antibody
      • Interleukin 1 receptor associated kinase 3 antibody
      • Interleukin 1 receptor associated kinase M antibody
      • Interleukin-1 receptor-associated kinase 3 antibody
      • IRAK M antibody
      • IRAK-3 antibody
      • IRAK-M antibody
      • Irak3 antibody
      • IRAK3_HUMAN antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: IRAKM knockout HAP1 cell lysate (20 µg)

      Lanes 1 - 2: Merged signal (red and green). Green - ab32394 observed at 69 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab32394 was shown to specifically react with IRAKM when IRAKM knockout samples were used. Wild-type and IRAKM knockout samples were subjected to SDS-PAGE. ab32394 and ab8245 (loading control to GAPDH) diluted to 1/5000 and 1/10000 respectively were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-IRAKM antibody [Y278] (ab32394) at 1/20000 dilution + A431 cell lysate

      Predicted band size: 68 kDa
      Observed band size: 68 kDa

    References

    ab32394 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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