Overview

  • Product nameAnti-IRE1 antibody
    See all IRE1 primary antibodies
  • Description
    Rabbit polyclonal to IRE1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide consisting of 16 amino acid from near the carboxy terminus (Human).

  • Positive control
    • WB: A20 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab37073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 107 kDa (predicted molecular weight: 110 kDa).
Please note there is a smaller isoform of IRE1 in rat (predicted molecular weight:68.6kDa; accession number is EDM06401). Block for 2 hours at RT or overnight at 4C using 5% non-fat milk.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionSenses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
  • Tissue specificityUbiquitously expressed. High levels observed in pancreatic tissue.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 KEN domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
    • Endoplasmic reticulum to nucleus signaling 1 antibody
    • Endoplasmic reticulum-to-nucleus signaling 1 antibody
    • Endoribonuclease antibody
    • ER to nucleus signaling 1 antibody
    • ERN 1 antibody
    • Ern1 antibody
    • ERN1_HUMAN antibody
    • hIRE 1p antibody
    • hIRE1p antibody
    • Inositol requiring 1 antibody
    • Inositol requiring 1, S. cerevisiae, homolog of antibody
    • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
    • Inositol requiring protein 1 antibody
    • inositol-requiring enzyme 1 antibody
    • Inositol-requiring protein 1 antibody
    • IRE 1 antibody
    • IRE 1a antibody
    • IRE 1P antibody
    • Ire1 alpha antibody
    • Ire1-alpha antibody
    • IRE1a antibody
    • Ire1alpha antibody
    • IRE1P antibody
    • MGC163277 antibody
    • MGC163279 antibody
    • Protein kinase/endoribonuclease antibody
    • RGD1559716 antibody
    • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
    see all

Anti-IRE1 antibody images

  • Lane 1 : Anti-IRE1 antibody (ab37073) at 0.5 µg/ml
    Lane 2 : Anti-IRE1 antibody (ab37073) at 1 µg/ml
    Lane 3 : Anti-IRE1 antibody (ab37073) at 2 µg/ml

    Lane 1 : A20 cell lysate
    Lane 2 : A20 cell lysate
    Lane 3 : A20 cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    goat anti-rabbit HRP at 1/10000 dilution

    Predicted band size : 110 kDa
    Observed band size : 107 kDa (why is the actual band size different from the predicted?)
  • ab37073, at 1 µg/ml, staining IRE1 in A20 cells with by ICC/IF.
  • Immunofluorescence of IRE1p in A20 cells using ab37073 at 2 ug/ml.

References for Anti-IRE1 antibody (ab37073)

This product has been referenced in:
  • Son SM  et al. Reduced IRE1a mediates apoptotic cell death by disrupting calcium homeostasis via the InsP3 receptor. Cell Death Dis 5:e1188 (2014). WB ; Human . Read more (PubMed: 24743743) »
  • Wang Y  et al. Inhibition of JNK by novel curcumin analog C66 prevents diabetic cardiomyopathy with a preservation of cardiac metallothionein expression. Am J Physiol Endocrinol Metab 306:E1239-47 (2014). Mouse . Read more (PubMed: 24714399) »

See all 10 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I will let you know as soon as I have any further information regarding the immunogen sequences fo...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (CEC)
Loading amount 30 µg
Specification CEC
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"