Synthetic peptide consisting of 16 amino acid from near the carboxy terminus (Human).
Our Abpromise guarantee covers the use of ab37073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 107 kDa (predicted molecular weight: 110 kDa).
Please note there is a smaller isoform of IRE1 in rat (predicted molecular weight:68.6kDa; accession number is EDM06401). Block for 2 hours at RT or overnight at 4C using 5% non-fat milk.
|ICC/IF||Use a concentration of 1 µg/ml.|
|ICC||Use a concentration of 1 µg/ml.|
ab37073, at 1 µg/ml, staining IRE1 in A20 cells with by ICC.
Immunocytochemistry/Immunofluorescence of IRE1p in A20 cells using ab37073 at 2 ug/ml.