Overview

  • Product name
  • Description
    Rabbit polyclonal to IRE1
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 15 amino acids near the N terminus of the human IRE1 protein.

  • Positive control
    • A20 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab37117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 107 kDa (predicted molecular weight: 110 kDa).Can be blocked with Human IRE1 peptide (ab39786).

Target

  • Function
    Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
  • Tissue specificity
    Ubiquitously expressed. High levels observed in pancreatic tissue.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 KEN domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
    • Endoplasmic reticulum to nucleus signaling 1 antibody
    • Endoplasmic reticulum-to-nucleus signaling 1 antibody
    • Endoribonuclease antibody
    • ER to nucleus signaling 1 antibody
    • ERN 1 antibody
    • Ern1 antibody
    • ERN1_HUMAN antibody
    • hIRE 1p antibody
    • hIRE1p antibody
    • Inositol requiring 1 antibody
    • Inositol requiring 1, S. cerevisiae, homolog of antibody
    • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
    • Inositol requiring protein 1 antibody
    • inositol-requiring enzyme 1 antibody
    • Inositol-requiring protein 1 antibody
    • IRE 1 antibody
    • IRE 1a antibody
    • IRE 1P antibody
    • Ire1 alpha antibody
    • Ire1-alpha antibody
    • IRE1a antibody
    • Ire1alpha antibody
    • IRE1P antibody
    • MGC163277 antibody
    • MGC163279 antibody
    • Protein kinase/endoribonuclease antibody
    • RGD1559716 antibody
    • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
    see all

Images

  • All lanes : Anti-IRE1 antibody (ab37117)

    Lane 1 : ab37117 at 0.5µg/ml + A20 cell lysate
    Lane 2 : ab37117 at 1µg/ml + A20 Cell lysate
    Lane 3 : ab37117 at 2µg/ml + A20 cell lysate


    Predicted band size : 110 kDa
    Observed band size : 107 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa,55 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence of IRE1p in A20 cells using ab37117 at 10 ug/ml.

References

ab37117 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab37117.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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