The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 107 kDa (predicted molecular weight: 110 kDa).Can be blocked with Human IRE1 peptide (ab39786).
FunctionSenses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
Tissue specificityUbiquitously expressed. High levels observed in pancreatic tissue.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 KEN domain. Contains 1 protein kinase domain.