Overview

  • Product nameAnti-IRE1 antibody
    See all IRE1 primary antibodies
  • Description
    Mouse monoclonal to IRE1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: EEVINLVDQT SENAPTTVSR DVEEKPAHAP ARPEAPVDSM LKDMATIILS TFLLIGWVAF IITYPLSMHQ QQQLQHQQFQ KELEKIQLLQ QQQQQLPFHP , corresponding to amino acids 401-500 of Human IRE1

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 110 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionSenses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
  • Tissue specificityUbiquitously expressed. High levels observed in pancreatic tissue.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 KEN domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
    • Endoplasmic reticulum to nucleus signaling 1 antibody
    • Endoplasmic reticulum-to-nucleus signaling 1 antibody
    • Endoribonuclease antibody
    • ER to nucleus signaling 1 antibody
    • ERN 1 antibody
    • Ern1 antibody
    • ERN1_HUMAN antibody
    • hIRE 1p antibody
    • hIRE1p antibody
    • Inositol requiring 1 antibody
    • Inositol requiring 1, S. cerevisiae, homolog of antibody
    • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
    • Inositol requiring protein 1 antibody
    • inositol-requiring enzyme 1 antibody
    • Inositol-requiring protein 1 antibody
    • IRE 1 antibody
    • IRE 1a antibody
    • IRE 1P antibody
    • Ire1 alpha antibody
    • Ire1-alpha antibody
    • IRE1a antibody
    • Ire1alpha antibody
    • IRE1P antibody
    • MGC163277 antibody
    • MGC163279 antibody
    • Protein kinase/endoribonuclease antibody
    • RGD1559716 antibody
    • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
    see all

Anti-IRE1 antibody images



  • Predicted band size : 110 kDa
    IRE1 antibody (ab54692) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab54692 at 10 µg/ml staining IRE1 in human Hela cells by Immunocytochemistry / Immunofluorescence.

References for Anti-IRE1 antibody (ab54692)

This product has been referenced in:
  • Ortega A  et al. Endoplasmic reticulum stress induces different molecular structural alterations in human dilated and ischemic cardiomyopathy. PLoS One 9:e107635 (2014). WB ; Human . Read more (PubMed: 25226522) »
  • Montalbano R  et al. Endoplasmic reticulum stress plays a pivotal role in cell death mediated by the pan-deacetylase inhibitor panobinostat in human hepatocellular cancer cells. Transl Oncol 6:143-57 (2013). WB, ICC/IF ; Human . Read more (PubMed: 23544167) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab54692.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"